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首页> 外文期刊>Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology >Molecular characterization of Kaposi's sarcoma associated herpesvirus (KSHV) from patients with AIDS-associated Kaposi's sarcoma in Sao Paulo, Brazil.
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Molecular characterization of Kaposi's sarcoma associated herpesvirus (KSHV) from patients with AIDS-associated Kaposi's sarcoma in Sao Paulo, Brazil.

机译：来自巴西圣保罗的与艾滋病相关的卡波西氏肉瘤患者的卡波西氏肉瘤相关疱疹病毒（KSHV）的分子表征。

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摘要

BACKGROUND:: Kaposi's sarcoma (KS) is caused by Kaposi's sarcoma associated herpesvirus (KSHV/HHV-8), the eighth Herpesvirus found to infect humans. The molecular epidemiology of KSHV is related closely to ethnicity and geographical location of studied populations. There is little epidemiological and molecular information about KSHV strains circulating in Brazil. OBJECTIVES:: To characterize KSHV strains isolated from AIDS patients with Kaposi's sarcoma (AIDS-KS) in Sao Paulo, Brazil, and to examine associations between KSHV subtypes, ethnicity and HIV risk categories. METHODS:: AIDS-KS patients were recruited consecutively at the largest AIDS reference hospital in Sao Paulo. Fragments (420bp) of the VR1 and VR2 regions of KSHV open reading frame (ORF) K1 were amplified by nested PCR and sequenced directly. RESULTS:: We analysed 37 samples from 33 patients, and found subtypes A-C in 48%, 21% and 30% of patients respectively, including two patients infected with subtype A5, a first report from Brazil. Sexual orientation was associated with subtype: 12/14 (86%) patients with subtype A were male homo/bisexual, compared with 3/8 (38%) among patients infected with subtype C (P=0.05). A higher proportion of male patients with subtype C were of Caucasian origin (7/8 (87%)), compared with 7/16 (44%) among male patients with subtype A (P=0.08). CONCLUSIONS:: This first detailed report of KSHV subtypes among AIDS-KS patients in Brazil reports the first isolation of KSHV subtype A5 in this country, and suggests KSHV strain transmission between different ethnic groups, and association of specific strains with sexual orientation.
机译：背景：卡波西氏肉瘤（KS）是由卡波西氏肉瘤相关疱疹病毒（KSHV / HHV-8）引起的，这是第八种感染人类的​​疱疹病毒。 KSHV的分子流行病学与所研究人群的种族和地理位置密切相关。关于在巴西流行的KSHV毒株的流行病学和分子信息很少。目的：鉴定从巴西圣保罗卡波西肉瘤（AIDS-KS）的艾滋病患者中分离出的KSHV株，并检查KSHV亚型，种族和HIV风险类别之间的关联。方法：在圣保罗最大的艾滋病参考医院连续招募AIDS-KS患者。通过巢式PCR扩增KSHV开放阅读框（ORF）K1的VR1和VR2区域的片段（420bp），并直接测序。结果：我们分析了33例患者的37个样本，分别在48％，21％和30％的患者中发现了A-C亚型，其中包括两名感染了A5亚型的患者，这是巴西的首次报道。性取向与亚型相关：A型亚型患者中有12/14（86％）是男性同性恋/双性恋，而C型亚型患者中有3/8（38％）（P = 0.05）。 C型亚型男性患者中，高加索人比例更高（7/8（87％）），而A型男性患者中男性比例为7/16（44％）（P = 0.08）。结论：这份关于巴西AIDS-KS患者中KSHV亚型的第一份详细报告报道了该国首次分离出KSHV A5亚型，并暗示了KSHV菌株在不同种族之间的传播，以及特定菌株与性取向的关联。

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