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首页> 外文期刊>Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology >Evaluation of the Loopamp((R)) (loop-mediated isothermal amplification) kit for detecting Norovirus RNA in faecal samples.
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Evaluation of the Loopamp((R)) (loop-mediated isothermal amplification) kit for detecting Norovirus RNA in faecal samples.

机译：对用于检测粪便样品中诺如病毒RNA的Loopamp（试剂盒）（环介导的等温扩增）试剂盒的评估。

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BACKGROUND: Noroviruses (NoVs) are associated with outbreaks of diarrhoeal illness in hospitals, nursing and residential homes and other institutional settings. NoV strains exhibit wide genetic diversity, and different virus genogroups and genotypes co-circulate in any geographical region at the same time, although most outbreaks of gastroenteritis are predominantly associated with genogroup II. The reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard for detecting NoVs in clinical samples. OBJECTIVES: This study evaluates commercialised Loopamp((R)) kits for detecting NoV GI and NoV GII in faecal samples collected from patients with gastroenteritis and compares the results with those obtained using real-time RT-PCR with NoV genogroup sequence-specific detection. STUDY DESIGN: Five hundred and ten faecal samples collected from patients with gastroenteritis were evaluated for the presence of NoV using the gold-standard real-time RT-PCRs and the Loopamp((R)) assays. RESULTS: The Loopamp((R)) Norovirus GI and GII detection kits performed well compared to genogroup-specific real-time RT-PCR. Although the sensitivity of detection of GI strains (83.3%) was less than that for GII strains (97.4%), this will have little impact on the laboratory diagnosis of NoV, since GII strains are associated with the majority of outbreaks examined. CONCLUSIONS: The Loopamp((R)) GII detection kit is a sensitive method for detecting all the commonly circulating GII-4 strains included in the evaluation panel.
机译：背景：诺如病毒（NoVs）与医院，疗养院和住宅以及其他机构环境中的腹泻病暴发有关。 NoV菌株表现出广泛的遗传多样性，不同的病毒基因组和基因型同时在任何地理区域内共同传播，尽管大多数胃肠炎的暴发主要与基因组II相关。逆转录聚合酶链反应（RT-PCR）是检测临床样品中NoV的金标准。目的：这项研究评估了用于检测胃肠炎患者粪便样本中NoV GI和NoV GII的商品化Loopamp（R）试剂盒，并将其结果与使用实时RT-PCR和NoV基因组序列特异性检测获得的结果进行了比较。研究设计：使用金标准实时RT-PCR和Loopamp（R）测定法评估了从胃肠炎患者收集的510份粪便样品中NoV的存在。结果：与基因组特异性实时R​​T-PCR相比，Loopamp（R）Norovirus GI和GII检测试剂盒表现良好。尽管对GI菌株的检测灵敏度（83.3％）低于对GII菌株（97.4％）的灵敏度，但这对NoV的实验室诊断几乎没有影响，因为GII菌株与所检查的大多数暴发有关。结论：Loopamp（R）GII检测试剂盒是一种灵敏的方法，可用于检测评估板中所有常见的GII-4菌株。

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