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首页> 外文期刊>Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology >Molecular evolution of HA gene of the influenza A H1N1 pdm09 strain during the consecutive seasons 2009-2011 in Hangzhou, China: Several immune-escape variants without positively selected sites
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Molecular evolution of HA gene of the influenza A H1N1 pdm09 strain during the consecutive seasons 2009-2011 in Hangzhou, China: Several immune-escape variants without positively selected sites

机译：2009-2011年连续的甲型H1N1流感pdm09流感病毒HA基因在中国杭州的分子进化：没有阳性选择位点的几种免疫逃逸变体

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Background: Even under immune pressure, the highly active influenza A H1N1 pdm09 variants emerged again in December 2010. Did the variability lead to poor vaccine effectiveness? Objectives: To study the genetic distance and antigenic drift of the influenza A H1N1 pdm09 strains based on the sequence analysis of HA virus gene segments during consecutive seasons 2009-2011 in Hangzhou, China. Study design: 39 Clinical samples from influenza-like-illness patients with culture-confirmed influenza A H1N1 pdm09 infections were collected over seasons in routine influenza surveillance. The HA gene was amplified and sequenced. A perspective analysis of genetic distance, antigenic drift and positively selected sites were conducted. Results: Analyses of human influenza A H1N1 pdm09 strains isolated in Hangzhou revealed that during the seasons 2009-2011, the antigenic drift had occurred, away from the northern hemisphere 2010/2011 influenza vaccine strain A/California/07/2009. The 2010/2011 viruses cluster in two main branches with a significant genetic distance, characterized by either S202T and S468N, or K180T/I, V216A, P288S, I312V and I389F. Interestingly, the epitopes corresponding to the immune-escape characteristic have altered much, but none of the amino acid substitutions in 2010/2011 variants were positively selected. Conclusions: The results of genetic surveillance in this study might account for frequent outbreaks of the influenza A H1N1 pdm09 strains since December 2010 and the disappearance after three months circulation. It facilitates early detection of antigenic sites for the virus to escape immunological restraint in 2010/2011 season. Continuous monitoring of antigenic changes is recommended.
机译：背景：即使在免疫压力下，高活性的甲型H1N1流感pdm09变异株也在2010年12月再次出现。这种变异性是否导致疫苗效果差？目的：基于2009-2011年连续两季HA病毒基因片段的序列分析，研究A型流感H1N1 pdm09株的遗传距离和抗原漂移。研究设计：在常规流感监测中，按季节收集了39例培养阳性的甲型H1N1 pdm09流感样流感患者的临床样本。 HA基因被扩增并测序。对遗传距离，抗原漂移和阳性选择位点进行了透视分析。结果：对在杭州分离出的人类甲型H1N1流感pdm09菌株的分析表明，在2009-2011年期间，抗原漂移发生在远离北半球2010/2011流感疫苗株A /加利福尼亚/ 07/2009的地方。 2010/2011病毒聚集在两个具有重要遗传距离的主要分支中，其特征是S202T和S468N或K180T / I，V216A，P288S，I312V和I389F。有趣的是，与免疫逃逸特征相对应的表位已发生很大变化，但没有积极选择2010/2011变体中的氨基酸取代。结论：这项研究的基因监测结果可能解释了自2010年12月以来甲型H1N1流感pdm09毒株的频繁暴发以及三个月流通后的消失。它有助于及早发现病毒的抗原位点，从而在2010/2011赛季摆脱免疫限制。建议连续监测抗原变化。

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