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首页> 外文期刊>Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology >Serologic assay for avian-origin influenza A (H7N9) virus in adults of Shanghai, Guangzhou and Yunnan, China
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Serologic assay for avian-origin influenza A (H7N9) virus in adults of Shanghai, Guangzhou and Yunnan, China

机译：上海，广州和云南成年人的禽源性甲型H7N9流感病毒血清学检测

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Background and objective: To investigate serologic status for novel avian influenza A (H7N9) virus in different areas in China, we examined serum samples collected in winter of 2011 from adult population of Shanghai (eastern China), Guangzhou (southern China) and Yunnan (southwest China) for the antibody responses to this virus. Study design: A total of 900 stored serum samples of adult outpatients (300 samples for each area) were subjected to anti-hemagglutinin (HA) antibodies assay using enzyme-linked immunosorbent assay (ELISA) and neutralizing antibodies assay using H7N9 pseudotyped particles (H7N9pp) and authentic H7N9 virus based neutralization test. Results: Anti-H7 antibodies were detected in 164, 186 and 123 samples from three areas above, respectively. Among anti-H7 positive sera, 20, 42 and 13 samples had neutralizing titers of 10 when 8×102 focus-forming units (FFU) of H7N9 pseudotyped particles (pp) were adopted in neutralizing assay, respectively. When neutralizing antibodies were assayed using classic microneutralization (MN) test, MN titers of 10 were found in 7 samples from Guangzhou, but none from Shanghai and Yunnan. Conclusion: Low levels of protective immunity pre-existed in some general adult population of the three areas, and pre-existing immunity against H7N9 in Guangzhou appears stronger than that in Shanghai and Yunnan.
机译：背景与目的：为了调查中国不同地区的新型甲型H7N9禽流感病毒的血清学状况，我们检查了2011年冬季从上海（华东），广州（华南）和云南（上海）的成年人群采集的血清样本中国西南）对该病毒的抗体反应。研究设计：使用酶联免疫吸附测定（ELISA）对总共900份成人门诊患者的血清样本（每个区域300份样本）进行抗血凝素（HA）抗体测定，并使用H7N9假型颗粒（H7N9pp）进行中和抗体测定）和基于真实的H7N9病毒的中和测试。结果：分别在以上三个区域的164、186和123个样本中检测到抗H7抗体。在抗H7阳性血清中，当在中和测定中采用H7N9假型颗粒（pp）的8×102焦点形成单位（FFU）时，分别有20、42和13个样品的中和效价> 10。当使用经典微中和（MN）试验分析中和抗体时，在广州的7个样品中发现MN滴度> 10，而上海和云南的样品中却没有。结论：在这三个地区的一些普通成年人中，低水平的保护性免疫力已经存在，而广州的H7N9免疫力似乎强于上海和云南。

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