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首页> 外文期刊>Journal of Comparative Pathology >Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus
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Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus

机译：感染Andrias davidianus Ranavirus的两栖和鱼类细胞系的形态变化

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摘要

Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe disease in Chinese giant salamanders, the largest extant amphibian in the world. A fish cell line, Epithelioma papulosum cyprinid (EPC), and a new amphibian cell line, Chinese giant salamander spleen cell (GSSC), were infected with ADRV and observed by light and electron microscopy. The morphological changes in these two cell lines infected with ADRV were compared. Cytopathic effect (CPE) began with rounding of the cells, progressing to cell detachment in the cell monolayer, followed by cell lysis. Significant CPE was visualized as early as 24 h post infection (hpi) in EPC cells and at 36 hpi in GSSC cells. Microscopical examination showed clear and significant CPE in EPC cells, while less extensive and irregular CPE with some adherent cells remaining was observed in GSSC cells. Following ADRV infection, CPE became more extensive. Transmission electron micrographs showed many virus particles around cytoplasmic vacuoles, formed as crystalline arrays or scattered in the cytoplasm of infected cells. Infected cells showed alteration in nuclear morphology, with condensed and marginalized nuclear chromatin on the inner aspect of the nuclear membrane and formation of a cytoplasmic viromatrix adjacent to the nucleus in both cell lines. Some virus particles were also detected in the nucleus of infected GSSC cells. Both cell lines are able to support replication of ADRV and can therefore be used to investigate amphibian ranaviruses. (C) 2015 Elsevier Ltd. All rights reserved.
机译：大卫·安达里亚斯鼻病毒（ADRV）是一种新兴的病毒病原体，它在世界上最大的现存两栖动物巨型giant中引起严重疾病。鱼细胞系，塞浦路斯上皮细胞（EPC）和新的两栖细胞系，中国巨型sal脾细胞（GSSC），被ADRV感染并通过光学和电子显微镜观察。比较了这两种被ADRV感染的细胞系的形态变化。细胞病变效应（CPE）从细胞变圆开始，发展到细胞单层中的细胞脱离，然后细胞裂解。早在EPC细胞中感染后24 h（hpi）以及GSSC细胞中36 hpi即可看到显着的CPE。显微镜检查显示EPC细胞中CPE明显且显着，而GSSC细胞中CPE的分布范围较广且不规则，且仍有一些贴壁细胞残留。在ADRV感染之后，CPE变得更加广泛。透射电子显微照片显示在细胞质液泡周围的许多病毒颗粒，形成为晶体阵列或散布在感染细胞的细胞质中。被感染的细胞显示出核形态的改变，在核膜的内部浓缩并边缘化了核染色质，并且在两个细胞系中都形成了邻近细胞核的胞质病毒基质。在感染的GSSC细胞核中也检测到一些病毒颗粒。两种细胞系均能够支持ADRV的复制，因此可用于研究两栖性蛙病毒。 （C）2015 Elsevier Ltd.保留所有权利。

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