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首页> 外文期刊>Journal of Comparative Pathology >Analysis of Cytokine Gene Expression using a Novel Chromogenic In-situ Hybridization Method in Pulmonary Granulomas of Cattle Infected Experimentally by Aerosolized Mycobacterium bovis
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Analysis of Cytokine Gene Expression using a Novel Chromogenic In-situ Hybridization Method in Pulmonary Granulomas of Cattle Infected Experimentally by Aerosolized Mycobacterium bovis

机译：新型发色原位杂交技术分析牛经气溶胶分枝杆菌感染的牛肺肉芽肿中细胞因子基因的表达

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Mycobacterium bovis is the cause of tuberculosis in most animal species including cattle and is a serious zoonotic pathogen. In man, M. bovis infection can result in disease clinically indistinguishable from that caused by Mycobacterium tuberculosis, the cause of most human tuberculosis. Regardless of host, the typical lesion induced by M. bovis or M. tuberculosis is the tuberculoid granuloma. Tuberculoid granulomas are dynamic structures reflecting the interface between host and pathogen and, therefore, pass through various morphological stages (I to IV). Using a novel in-situ hybridization assay, transcription of various cytokine and chemokine genes was examined qualitatively and quantitatively using image analysis. In experimentally infected cattle, pulmonary granulomas of all stages were examined 150 days after aerosol exposure to M. bovis. Expression of mRNA encoding tumour necrosis factor (TNF)-alpha, transforming growth factor-beta, interferon (IFN)-gamma, interleukin (IL)-17A, IL-16, IL-10, CXCL9 and CXCL10 did not differ significantly between granulomas of different stages. However, relative expression of the various cytokines was characteristic of a Th1 response, with high TNF-alpha and IFN-gamma expression and low IL-10 expression. Expression of IL-16 and the chemokines CXCL9 and CXCL10 was high, suggestive of granulomas actively involved in T-cell chemotaxis. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.orgflicenses/by-nc-nd/4.0/).
机译：牛分枝杆菌是包括牛在内的大多数动物物种中结核病的病因，并且是严重的人畜共患病原体。在人中，牛分枝杆菌感染可导致与由结核分枝杆菌引起的疾病在临床上无法区分，结核分枝杆菌是大多数人类结核病的病因。不论宿主如何，牛分枝杆菌或结核分枝杆菌引起的典型病变是结核性肉芽肿。结核性肉芽肿是反映宿主和病原体之间界面的动态结构，因此会经历各种形态学阶段（I至IV）。使用新颖的原位杂交测定，使用图像分析定性和定量地检查了各种细胞因子和趋化因子基因的转录。在经实验感染的牛中，气溶胶暴露于牛分枝杆菌后150天检查所有阶段的肺肉芽肿。肉芽肿之间的编码肿瘤坏死因子（TNF）-α，转化生长因子-β，干扰素（IFN）-γ，白介素（IL）-17A，IL-16，IL-10，CXCL9和CXCL10的mRNA表达没有明显差异不同阶段。但是，各种细胞因子的相对表达是Th1反应的特征，TNF-α和IFN-γ的表达较高，而IL-10的表达较低。 IL-16和趋化因子CXCL9和CXCL10的表达较高，提示肉芽肿活跃地参与了T细胞的趋化性。由Elsevier Ltd.发布。这是CC BY-NC-ND许可下的开放获取文章（http：//creativecommons.orgflicenses/by-nc-nd/4.0/）。

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