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首页> 外文期刊>Journal of Comparative Pathology >CD8-positive Lymphocytes in Graft-Versus-Host Disease of Humanized NOD
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CD8-positive Lymphocytes in Graft-Versus-Host Disease of Humanized NOD

机译：人源化NOD移植物抗宿主病中的CD8阳性淋巴细胞

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Immunocompromised mice that can support a human immune system are an increasingly important model for the investigation of haemopoietic stem/progenitor cell (HSPC) development and human infectious disease. NOD-SCID IL-2R gamma(-/-) (NSG) mice engrafted with human fetal liver and thymus prior to HSPC engraftment, commonly known as NSG bone marrow liver thymus (NSG-hu-BLT) mice, are one such model and have robust reconstitution of human leucocytes within the peripheral blood and tissues. Four NSG-hu-BLT mice were submitted for diagnostic necropsy examination following the development of alopecia, pruritus and lethargy after HSPC engraftment. Histopathology revealed multifocal to coalescing single keratinocyte cell death in the epidermis and follicles with dermatitis and mild dermal fibrosis. Single-cell hepatocyte cell death was present in three cases, with various degrees of portal fibrosis. In the skin and liver, cell death was associated with lymphocytes that reacted with anti-human CD45, CD3 and CD8 antibodies, consistent with a diagnosis of graft-versus-host disease (GvHD). This study expands on recently reported microscopical features of GvHD in NSG-hu-BLT mice and suggests a role for CD8(+) T lymphocytes in the progression of the disease. NSG-hu-BLT mice represent an excellent model of GvHD, but its prevalence may compromise their use in other fields of biomedical research. (C) 2015 Elsevier Ltd. All rights reserved.
机译：可以支持人类免疫系统的免疫功能低下的小鼠对于研究造血干/祖细胞（HSPC）的发育和人类感染性疾病越来越重要。在HSPC植入之前将人胎肝和胸腺植入的NOD-SCID IL-2Rγ（-/-）（NSG）小鼠就是这样一种模型，通常称为NSG骨髓肝胸腺（NSG-hu-BLT）小鼠。在外周血和组织中具有强大的人类白细胞重构能力。 HSPC植入后，将四只NSG-hu-BLT小鼠提交用于脱发，瘙痒和嗜睡的诊断性尸检。组织病理学显示表皮和卵泡中皮炎和轻度皮肤纤维化多灶性至合并单个角质形成细胞死亡。三例出现单细胞肝细胞死亡，并伴有不同程度的门脉纤维化。在皮肤和肝脏中，细胞死亡与与抗人CD45，CD3和CD8抗体反应的淋巴细胞有关，这符合对移植物抗宿主病（GvHD）的诊断。这项研究扩展了NSG-hu-BLT小鼠中最近报道的GvHD的微观特征，并暗示了CD8（+）T淋巴细胞在疾病进展中的作用。 NSG-hu-BLT小鼠代表了GvHD的出色模型，但其流行程度可能会损害其在生物医学研究的其他领域的使用。 （C）2015 Elsevier Ltd.保留所有权利。

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