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首页> 外文期刊>Journal of Comparative Pathology >Lack of Correlation between Mucosal Immunoglobulin A-positive Plasma Cell Numbers and TLR5 Genotypes in German Shepherd Dogs with Idiopathic Chronic Enteropathy
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Lack of Correlation between Mucosal Immunoglobulin A-positive Plasma Cell Numbers and TLR5 Genotypes in German Shepherd Dogs with Idiopathic Chronic Enteropathy

机译：特发性慢性肠病的德国牧羊犬的粘膜免疫球蛋白A阳性浆细胞数量与TLR5基因型之间缺乏相关性

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摘要

It has been suggested previously that a deficiency in mucosal immunoglobulin (Ig) A production could be involved in the pathogenesis of chronic enteropathy in German shepherd dogs (GSDs). Recent research has shown that single nucleotide polymorphisms in the gene encoding Toll-like receptor (TLR)-5 are associated with an increased risk of development of chronic idiopathic enteropathy in this breed. IgA is essential for mucosal immunity and studies in mice have linked the interaction of TLR5 with its ligand flagellin to class switching of B cells into IgA-producing plasma cells. We hypothesized that dogs carrying the risk-associated (RA) genotypes for G22A and C100T genes of TLR5 would have a different number of IgA plasma cells in the duodenal and colonic mucosa compared with dogs carrying the risk-protective (RP) genotypes. Thirty-one GSDs were diagnosed with idiopathic chronic enteropathy by clinical exclusion diagnosis and histopathological confirmation. Immunohistochemistry was performed using goat anti-dog IgA primary antibody. Two sections of duodenum, and colon if available, were examined from each animal. Twelve images were captured from each section and IgA-positive cells were counted and expressed per 10,000 mu m(2). TLR5 genotypes for the G22A and C100T genes were determined by polymerase chain reaction on blood samples. Numbers of IgA-positive cells in the duodenum and colon were slightly higher than those published previously for GSDs with or without chronic enteropathy (mean in the crypt area of the duodenum 52.6 +/- 16.2; mean in the tip of the duodenal villus 51.12 +/- 3.83; mean in the base of the duodenal villus 55.02 +/- 3.3; mean in the crypt area of the colon 67.4 +/- 4.3). There was no correlation between numbers of IgA-positive cells in duodenum or colon between dogs carrying the RA versus the RP alleles of TLR genes. Further studies are needed to assess the production of secretory IgA and its relationship to TLR5 genotypes. (C) 2015 Elsevier Ltd. All rights reserved.
机译：以前曾有人提出，德国牧羊犬（GSD）慢性肠病的发病机理可能与粘膜免疫球蛋白（Ig）A的缺乏有关。最近的研究表明，在该品种中，编码Toll样受体（TLR）-5的基因中的单核苷酸多态性与罹患慢性特发性肠病的风险增加有关。 IgA对于粘膜免疫至关重要，并且在小鼠中的研究已经将TLR5与其配体鞭毛蛋白的相互作用与B细胞向产生IgA的浆细胞的类别转换联系起来。我们假设，携带TLR5 G22A和C100T基因风险相关（RA）基因型的狗与携带风险保护（RP）基因型的狗相比，十二指肠和结肠黏膜中IgA浆细胞的数量不同。通过临床排除诊断和组织病理学证实，有31例GSD被诊断为特发性慢性肠病。使用山羊抗狗IgA一抗进行免疫组织化学。从每只动物检查十二指肠和结肠（如果有）的两个部分。从每个部分捕获十二个图像，并且每10,000微米（2）计数和表达IgA阳性细胞。通过对血液样本进行聚合酶链反应确定G22A和C100T基因的TLR5基因型。十二指肠和结肠中IgA阳性细胞的数量略高于先前公布的有或没有慢性肠病的GSD的数量（平均值在十二指肠隐窝区域52.6 +/- 16.2;平均值在十二指肠绒毛尖端51.12 + /-3.83;平均值在十二指肠绒毛的底部55.02 +/- 3.3;平均值在结肠的隐窝区域67.4 +/- 4.3）。在携带RA的狗与TLR基因的RP等位基因之间，十二指肠或结肠中IgA阳性细胞数量之间没有相关性。需要进一步的研究来评估分泌型IgA的产生及其与TLR5基因型的关系。 （C）2015 Elsevier Ltd.保留所有权利。

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