• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical Biochemistry >Polyelectrolyte-stabilized glucose biosensor based on woodceramics as electrode.
【24h】

Polyelectrolyte-stabilized glucose biosensor based on woodceramics as electrode.

机译：以木陶瓷为电极的聚电解质稳定型葡萄糖生物传感器。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

OBJECTIVE: Different biosensors for early diagnosis of diabetes have been discussed increasingly. We demonstrate the applicability of a glucose biosensor for rapid and quantitative determination of glucose in experimental sample solutions.DESIGN AND METHODS: Polyethylenimine (PEI)-stabilized glucose oxidase (GOD) was used for the construction of stable glucose biosensor utilizing a simple manufacture procedure. The stabilized glucose oxidase was immobilized by physical adsorption into activated porous and conductive carbonaceous material-woodceramics that served as the immobilization matrix and the electrochemical transducer. The optimum parameters for the construction of the biosensor and the influences of pH and temperature on the biosensor response were explored.RESULTS: Optimum results were obtained using soaking solutions of 2300 U/ml GOD, 10 mg/ml mediator toluidine blue O (TBO), and 2.0%, w/v PEI. The sensitivity of the resulting biosensor for glucose was 1.22 microA/mM, with a linear response in the concentration range of 0.5-7 mM. The sensor-to-sensor reproducibility was good, the relative standard deviation (R.S.D.) being less than 8.0%. The storage and operational stabilities of the glucose biosensor were good.CONCLUSIONS: The glucose biosensor provides reproducible and quantitative information on glucose within minutes and is applicable for the detection of glucose in experimental samples.
机译：目的：越来越多地讨论了用于糖尿病早期诊断的不同生物传感器。我们证明了一种葡萄糖生物传感器可用于快速定量测定实验样品溶液中的葡萄糖。设计与方法：聚乙烯亚胺（PEI）稳定的葡萄糖氧化酶（GOD）用于通过简单的制造过程构建稳定的葡萄糖生物传感器。通过物理吸附将稳定的葡萄糖氧化酶固定在活化的多孔和导电碳质材料-木陶瓷中，该陶瓷用作固定基质和电化学换能器。结果表明：采用2300 U / ml GOD，10 mg / ml介质甲苯胺蓝O（TBO）浸泡溶液可获得最佳结果。和2.0％（含PEI）。所得生物传感器对葡萄糖的灵敏度为1.22 microA / mM，线性响应在0.5-7 mM的浓度范围内。传感器之间的可重复性良好，相对标准偏差（R.S.D.）小于8.0％。结论：该葡萄糖生物传感器可在数分钟内提供葡萄糖的可再现和定量信息，可用于检测实验样品中的葡萄糖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号