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首页> 外文期刊>Journal of Comparative Physiology, B. Biochemical, Systemic, and Environmental Physiology >Antifreeze proteins in the Antarctic springtail, Gressittacantha terranova
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Antifreeze proteins in the Antarctic springtail, Gressittacantha terranova

机译：南极跳尾羚中的抗冻蛋白

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摘要

Antarctic springtails are exemplars of extreme low temperature adaptation in terrestrial arthropods. This paper represents the first examination of such adaptation in the springtail, Gressittacantha terranova. Acclimatization state was measured in field-fresh samples over a 22-day period at the beginning of the austral summer. No evidence of temperature tracking was observed. Mean temperature of crystallization (T (c)) for all samples was -20.67 +/- A 0.32A degrees C and the lowest T (c) recorded was -32.62A degrees C. Ice affinity purification was used to collect antifreeze proteins (AFPs) from springtail homogenate. The purified ice fraction demonstrated both thermal hysteresis activity and recrystallisation inhibition. Growth-melt observations revealed that ice crystals grow normal to the c-axis (basal plane). Reverse-phased HPLC produce one clearly resolved peak (P1) and one compound peak (P2). Mass spectrometry identified the molecular mass of P1 as 8,599 Da. The P1 protein was also the most prominent in P2, although additional peptides of 6-7 KDa were also prominent. The main AFP of the Antarctic springtail, G. terranova has been isolated, although like other AFP-expressing arthropods, it shows evidence of expressing a family of AFPs.
机译：南极跳马是陆生节肢动物中极低温度适应性的典范。本文代表了对这种适应性在跳尾类动物Gressittacantha terranova中的首次研究。在南方夏季开始的22天内，对田间新鲜样品的驯化状态进行了测量。没有观察到温度跟踪的证据。所有样品的平均结晶温度（T（c））为-20.67 +/- A 0.32A摄氏度，记录的最低T（c）为-32.62A摄氏度。使用冰亲和纯化技术收集抗冻蛋白（AFP） ）从弹尾匀浆。纯化的冰级分显示出热滞活性和重结晶抑制作用。生长融化的观察表明，冰晶垂直于c轴（基面）生长。反相HPLC产生一个清晰分辨的峰（P1）和一个化合物峰（P2）。质谱鉴定出P1的分子量为8,599 Da。 P1蛋白在P2中也最突出，尽管其他6-7 KDa肽也很突出。尽管与其他表达AFP的节肢动物一样，它也分离出了南极跳马的主要AFP，但它显示出表达AFP家族的证据。

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