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首页> 外文期刊>Journal of clinical neuroscience: official journal of the Neurosurgical Society of Australasia >Immunohistochemical profile of transforming growth factor-beta1 and basic fibroblast growth factor in sciatic nerve anastomosis following pinealectomy and exogenous melatonin administration in rats.
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Immunohistochemical profile of transforming growth factor-beta1 and basic fibroblast growth factor in sciatic nerve anastomosis following pinealectomy and exogenous melatonin administration in rats.

机译：松果体切除术和外源性褪黑激素给药后大鼠坐骨神经吻合中转化生长因子-β1和碱性成纤维细胞生长因子的免疫组织化学谱。

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摘要

Collagen scar formation at the cut end of a peripheral nerve, an important problem in clinical practice for neurosurgeons, obstructs sprouting of axons into appropriate distal fascicles, and thereby limits the regeneration process. Researchers have attempted to control collagen accumulation and neuroma formation with various physical and chemical methods, but with limited functional success. Recently, it has been demonstrated that transforming growth factor (TGF)-beta and basic fibroblast growth factor (bFGF) play an important role in collagen production by fibroblasts and in Schwann cell activity. In our study, rats were divided into a control group, a melatonin-treated group, a surgical pinealectomy group, and a group treated with melatonin following pinealectomy. They then underwent a surgical sciatic nerve transection and primary suture anastomosis. At 2 months after anastomosis, the animals were sacrificed and unilateral sciatic nerve specimens, including the anastomotic region, were removed and processed for immunohistochemical study from two animals in each group. For each antibody, immunoreactivity was assessed using a semiquantitative scoring system. Strong TGF-beta1 and/or bFGF expression was observed in the epineurium of animals that underwent pinealectomy, but no or weak staining was observed in animals in the control and melatonin treatment groups. Based on these data, we suggest that both TGF-beta1 and bFGF have important roles in control of collagen accumulation and neuroma formation at the anastomotic site, and that the pineal neurohormone melatonin has a beneficial effect on nerve regeneration.
机译：胶原蛋白疤痕在周围神经的切割端形成，这是神经外科医师在临床实践中的一个重要问题，阻碍了轴突发芽到适当的远端束中，从而限制了再生过程。研究人员已尝试通过各种物理和化学方法来控制胶原蛋白的积累和神经瘤的形成，但在功能上的成功有限。最近，已经证明转化生长因子（TGF）-β和碱性成纤维细胞生长因子（bFGF）在成纤维细胞产生胶原蛋白和雪旺氏细胞活性中起重要作用。在我们的研究中，将大鼠分为对照组，褪黑素治疗组，外科松果体切除术组和松果体切除术后接受褪黑素治疗的组。然后，他们进行了手术坐骨神经横切术和原发性缝合线吻合术。吻合后2个月，处死动物，取出单侧坐骨神经标本，包括吻合区，并从每组的两只动物中进行免疫组织化学研究。对于每种抗体，使用半定量评分系统评估免疫反应性。在接受松果体切除术的动物的神经外膜中观察到强的TGF-β1和/或bFGF表达，但在对照组和褪黑素治疗组的动物中未观察到或仅有弱染色。基于这些数据，我们认为TGF-beta1和bFGF在吻合部位的胶原蛋白积累和神经瘤形成的控制中都具有重要作用，而松果体神经激素褪黑激素对神经再生具有有益作用。

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