首页> 外文期刊>Journal of clinical neuroscience: official journal of the Neurosurgical Society of Australasia >A simple behavioral test for locomotor function after brain injury in mice.
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A simple behavioral test for locomotor function after brain injury in mice.

机译:小鼠脑损伤后运动功能的简单行为测试。

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To establish a simple and reliable test for assessing locomotor function in mice with brain injury, we developed a new method, the rotarod slip test, in which the number of slips of the paralytic hind limb from a rotarod is counted. Brain injuries of different severity were created in adult C57BL/6 mice, by inflicting 1-point, 2-point and 4-point cryo-injuries. These mice were subjected to the rotarod slip test, the accelerating rotarod test and the elevated body swing test (EBST). Histological analyses were performed to assess the severity of the brain damage. Significant and consistent correlations between test scores and severity were observed for the rotarod slip test and the EBST. Only the rotarod slip test detected the mild hindlimb paresis in the acute and sub-acute phase after injury. Our results suggest that the rotarod slip test is the most sensitive and reliable method for assessing locomotor function after brain damage in mice.
机译:为了建立评估脑损伤小鼠运动功能的简单而可靠的测试方法,我们开发了一种新的方法,即旋转脚踏试验,该方法计算了从旋转脚踏车中麻痹后肢的滑动次数。通过造成1点,2点和4点冷冻伤害,在成年C57BL / 6小鼠中造成了不同严重程度的脑损伤。对这些小鼠进行了旋转脚架滑移测试,加速的旋转脚架测试和升高的身体摆动测试(EBST)。进行组织学分析以评估脑损伤的严重性。对于旋转脚架滑移测试和EBST,观察到分数和严重性之间的显着且一致的相关性。只有旋转脚踏试验在受伤后的急性和亚急性期检测到轻度后肢轻瘫。我们的结果表明,旋转脚踏打滑试验是评估小鼠脑损伤后运动功能的最灵敏,最可靠的方法。

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