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首页> 外文期刊>Journal of Comparative Physiology, B. Biochemical, Systemic, and Environmental Physiology >Fatty acid esterification in the yolk sac membrane of the avian embryo
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Fatty acid esterification in the yolk sac membrane of the avian embryo

机译：禽胚胎卵黄囊膜中的脂肪酸酯化

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摘要

The transfer of lipid from the yolk to the avian embryo is mediated by the yolk sac membrane (YSM). Some, but not all, of the published morphological evidence supports the view that the lipid undergoes a cycle of hydrolysis and re-esterification during translocation across the YSM. The present study aims to test this view by investigating the capacity of the YSM to perform esterification of free fatty acids to form acyl-lipids. YSM pieces (area vasculosa), obtained from the chicken embryo at day 10 of development, were incubated in vitro in medium containing [C-14]-palmitic acid. Radioactivity was rapidly incorporated into the tissue lipid indicating a high capacity for esterification. The incorporation was linear with time during the 1-h incubation. Approximately 84% of the incorporated label was recovered in triacylglycerol, 12% was incorporated into phospholipid and less than 1% was detected in cholesteryl ester. [C-14]-palmitic acid was incorporated primarily at the sn-1/3 positions in the triacylglycerol molecule and at the sn-1 position of phospholipid. The incorporation of label into tissue pieces obtained from the non-vascularized peripheral region of the YSM (area vitellina) was much more limited than that observed for the area vasculosa. The results support the hypothesis that yolk lipid is hydrolyzed and re-esterified during transfer across the YSM.
机译：卵黄囊膜（YSM）介导脂质从卵黄到禽类胚胎的转移。一些（但不是全部）已公开的形态学证据支持这样的观点，即脂质在跨YSM转运过程中经历了水解和重新酯化的循环。本研究旨在通过研究YSM进行游离脂肪酸酯化以形成酰基脂质的能力来检验这种观点。在发育的第10天从鸡胚获得的YSM片（血管区域）在含有[C-14]-棕榈酸的培养基中进行体外培养。放射性迅速掺入组织脂质中，表明具有很高的酯化能力。在1小时的孵育过程中，掺入量与时间呈线性关系。在三酰基甘油中回收了约84％的掺入标记，在磷脂中掺入了12％，在胆固醇酯中检测到的不足1％。 [C-14]-棕榈酸主要掺入三酰基甘油分子的sn-1 / 3位置和磷脂的sn-1位置。与从YSM的非血管化的周边区域（卵黄质区域）获得的组织碎片中掺入标签相比，对血管区域所观察到的限制更大。该结果支持以下假设：蛋黄脂质在跨YSM的转移过程中会水解并重新酯化。

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