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首页> 外文期刊>Journal of Clinical Immunology >CCR7+ central and CCR7- effector memory CD4 + T cells in human cutaneous Leishmaniasis.
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CCR7+ central and CCR7- effector memory CD4 + T cells in human cutaneous Leishmaniasis.

机译：人皮肤利什曼病中的CCR7 +中枢和CCR7-效应器记忆CD4 + T细胞。

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The profile of central (=T(CM)) and effector (=T(EM)) memory CD4(+) T cell subsets and the possible role as surrogate markers of protection is studied in the volunteers with history of cutaneous leishmaniasis (HCL).Profile of T cell subsets based on CCR7/CD45RA expressions and phenotypic changes after soluble Leishmania antigen (SLA) stimulation were analyzed. Then, sorted CD4(+)CD45RO(-)CD45RA(+) na?ve T, CD4(+)CD45RO(+)CD45RA(-)CCR7(-) T(EM,) CD4(+)CD45RO(+)CD45RA(-)CCR7(+) T(CM) subsets were cultured with SLA for proliferation, cytokine production and intracellular cytokine assays.In the HCL and control volunteers, the mean frequencies of CD4(+)CD45RA(+)CCR7(+) na?ve T cells and CD4(+)CD45RA(-)CCR7(-) T(EM) cells were higher than the other subsets before culture. Frequency of na?ve T cells and CD4(+)CD45RA(-)CCR7(+) T(CM) cells was significantly decreased (P=0.01 for na?ve T and P<0.05 for T(CM) cells) and frequency of T(EM) cells was significantly increased after SLA stimulation compared to before culture (P<0.001). By CFSE labeling, CD4(+)CD45RO(+)CD45RA(-)CCR7(+) T(CM) cells showed more proliferation potential than CD4(+)CD45RO(+)CD45RA(-)CCR7(-) T(EM) cells. Stimulation of the T(EM) cells in HCL volunteers induced a significantly higher IFN-γ production (P=0.04) with higher number of intracellular IFN-γ positive cells (P=0.032) than the same cells from controls. A significantly higher number of T(CM) cells produced IL-2 in HCL volunteers compared with controls (P<0.05). Most of the intracellular IFN-γ positive T(EM) cells were proliferating CFSE-dim populations (P<0.05).A combination of Leishmania-reactive IFN-γ producing CD4(+)CD45RO(+)CD45RA(-)CCR7(-) T(EM) and Leishmania-reactive IL-2 producing CD4(+)CD45RO(+)CD45RA(-)CCR7(+) T(CM) are identified in individuals with history of CL which might play a role in protective recall immune response against Leishmania infection.
机译：在有皮肤利什曼病病史的志愿者中研究了中枢（= T（CM））和效应子（= T（EM））记忆CD4（+）T细胞亚群的概况以及作为保护替代标志物的可能作用分析了可溶的利什曼原虫抗原（SLA）刺激后基于CCR7 / CD45RA表达和表型变化的T细胞亚群概况。然后，对CD4（+）CD45RO（-）CD45RA（+）原始T进行排序，CD4（+）CD45RO（+）CD45RA（-）CCR7（-）T（EM，）CD4（+）CD45RO（+）CD45RA （-）CCR7（+）T（CM）亚群与SLA培养，用于增殖，细胞因子生成和细胞内细胞因子测定。在HCL和对照志愿者中，CD4（+）CD45RA（+）CCR7（+）na的平均频率五个T细胞和CD4（+）CD45RA（-）CCR7（-）T（EM）细胞高于培养前的其他亚群。幼稚T细胞和CD4（+）CD45RA（-）CCR7（+）T（CM）细胞的频率显着降低（幼稚T的P = 0.01，T（CM）细胞的P <0.05）和频率与培养前相比，SLA刺激后T（EM）细胞的数量显着增加（P <0.001）。通过CFSE标记，CD4（+）CD45RO（+）CD45RA（-）CCR7（+）T（CM）细胞显示出比CD4（+）CD45RO（+）CD45RA（-）CCR7（-）T（EM）更多的增殖潜能细胞。在HCL志愿者中刺激T（EM）细胞诱导了明显更高的IFN-γ产生（P = 0.04），其中细胞内IFN-γ阳性细胞的数量也高于对照组的相同细胞（P = 0.032）。与对照组相比，HCL志愿者中产生IL-2的T（CM）细胞数量明显增加（P <0.05）。大多数细胞内IFN-γ阳性T（EM）细胞均增殖为CFSE-dim种群（P <0.05）。利什曼原虫反应性IFN-γ组合产生CD4（+）CD45RO（+）CD45RA（-）CCR7（- T（EM）和利什曼原虫反应性IL-2产生CD4（+）CD45RO（+）CD45RA（-）CCR7（+）T（CM）在具有CL病史的个体中被鉴定出，可能在保护性召回免疫中发挥作用对利什曼原虫感染的反应。

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