...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Chromatographic Science >Separation and purification of rutin and acaciin from the chinese medicinal herb herba cirsii by combination of macroporous absorption resin and high-speed counter-current chromatography
【24h】

Separation and purification of rutin and acaciin from the chinese medicinal herb herba cirsii by combination of macroporous absorption resin and high-speed counter-current chromatography

机译：大孔吸附树脂与高速逆流色谱法相结合分离纯化中药材中芦丁和阿拉伯胶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of rutin and acaciin from the Chinese medicinal herb Herba Cirsii was successfully established. The crude extracts obtained from Herba Cirsii by water under reflux were subjected to a macroporous resin column and eluted with 10% and 60% ethanol, respectively. The fraction of 60% ethanol was used as the sample for HSCCC separation of rutin and acaciin. The two-phase solvent system used for the separation was ethyl acetate-n-butanol-water (5:1.5:5, v/v) and the upper phase was used as the stationary phase. Rutin (25.2 mg) and acaciin (21.8 mg) with a purity of 99.2% and 99.6%, respectively, were purified successfully from 60 mg of sample. The chemical structures of rutin and acaciin were identified by 1H-NMR and 13C-NMR.
机译：成功建立了一种从中药草中分离和纯化芦丁和阿拉伯胶的制备型高速逆流色谱法。将通过水在回流下从赫氏草获得的粗提取物置于大孔树脂柱上，并分别用10％和60％的乙醇洗脱。将60％的乙醇部分用作HSCCC分离芦丁和阿拉伯胶的样品。用于分离的两相溶剂体系是乙酸乙酯-正丁醇-水（5：1.5：5，v / v），上层相用作固定相。从60 mg样品中成功纯化出分别为99.2％和99.6％纯度的芦丁（25.2 mg）和阿拉伯胶（21.8 mg）。芦丁和阿拉伯胶的化学结构通过1 H-NMR和13 C-NMR鉴定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号