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首页> 外文期刊>Journal of Chromatographic Science >An Ultra-Performance Liquid Chromatography Photodiode Array Detection Tandem Mass Spectrometric Method for Simultaneous Determination of Seven Major Bioactive Constituents in Xiaochaihutang and Its Application to Fourteen Compatibilities Study
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An Ultra-Performance Liquid Chromatography Photodiode Array Detection Tandem Mass Spectrometric Method for Simultaneous Determination of Seven Major Bioactive Constituents in Xiaochaihutang and Its Application to Fourteen Compatibilities Study

机译：同时测定小柴胡汤中七种主要生物活性成分的超高效液相色谱光电二极管阵列检测串联质谱法及其在十四种相容性研究中的应用

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摘要

A rapid and sensitive ultra-performance liquid chromatography photodiode array detection tandem mass spectrometric method (UPLC-PDA-MS-MS) was developed and validated to simultaneously determine seven major bioactive constituents in the formula of traditional Chinese medicines Xiaochaihutang (XCHT). To investigate the discipline of compatibility in XCHT, 14 kinds of compatibilities designed by orthogonal array were also analyzed. The separation was performed on an ACQUITY UPLC (TM) BEH C-18 column (100 x 2.1 mm, 1.7 mu m) using gradient elution with a mobile phase of 0.1% formic acid and acetonitrile at a flow rate of 0.2 mL/min. Two detection techniques of PDA detector and MS-MS detector were proposed, respectively. The concentrations of baicalin and wogono-side were high enough for PDA detection while low-concentration bioactive constituents including saikosaponin a, ginsenoside Rg1, liquiritin, baicalein and wogonin were quantified by MS-MS detection. The proposed method was fully validated in terms of sensitivity, linearity, specificity, precision, repeatability and recovery. This is the first report on the simultaneous determination of the major bioactive constituents of XCHT by UPLC-PDA-MS-MS, which could be used to evaluate the quality of XCHT and to investigate the discipline of compatibility in XCHT.
机译：建立了快速灵敏的超高效液相色谱光电二极管阵列检测串联质谱法（UPLC-PDA-MS-MS），该方法可同时测定中草药小柴胡汤（XCHT）配方中的7种主要生物活性成分。为了研究XCHT中的兼容性原则，还分析了通过正交阵列设计的14种兼容性。分离是在ACQUITY UPLC TM BEH C-18色谱柱（100 x 2.1 mm，1.7μm）上进行的，使用0.1％甲酸和乙腈的流动相进行梯度洗脱，流速为0.2 mL / min。分别提出了PDA检测器和MS-MS检测器的两种检测技术。黄ical苷和Wogono侧的浓度足以用于PDA检测，而低浓度生物活性成分（包括皂苷A，人参皂苷Rg1，Liquiritin，黄ical苷和Wogonin）通过MS-MS检测进行定量。该方法在灵敏度，线性，特异性，精密度，重复性和回收率方面均得到了充分验证。这是有关通过UPLC-PDA-MS-MS同时测定XCHT主要生物活性成分的第一份报告，可用于评估XCHT的质量并研究XCHT的相容性。

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