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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis
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Assay of free ferulic acid and total ferulic acid for quality assessment of Angelica sinensis

机译：游离阿魏酸和总阿魏酸含量测定当归质量的方法

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摘要

Activity of Chinese Danggui (DG), the processed root of Angelica sinensis (Oliv.) Diels, is linked to the ferulic acid content but the stability of ferulic acid during extraction for medicinal use is not known. The stabilities of ferulic acid and coniferyl ferulate were evaluated in the extracts of DG using a variety of extraction solvents. These included various combinations and proportions of methanol, water, formic acid, I M aqueous hydrochloric acid and 2% sodium hydrogen carbonate (NaHCO3) in water. Coniferyl ferulate was found liable to hydrolyze into ferulic acid in neutral, strongly acidic and basic solvents, where heat and water could facilitate this hydrolysis. However, the hydrolysis was relatively resisted in weakly organic acid. Based on the stability evaluation, two new terms, namely: free ferulic acid and total ferulic acid, were suggested and defined. Free ferulic acid refers to the natural content of ferulic acid in herbs. Total ferulic acid means the sum of free ferulic acid plus the amount of related hydrolyzed components. Meanwhile, the high-performance liquid chromatographic (HPLC) method was developed to assay free ferulic acid and total ferulic acid in DG using methanol-formic acid (95:5) and methanol-2% NaHCO3 in water (95:5) as extraction solvents, respectively. Ten DG samples were investigated on their contents of free and total ferulic acid. The results indicated that the amount variety of free ferulic acid was larger than that of their counterparts, and the ratio of total ferulic acid to free ferulic acid was 4.07 +/- 2.73 (mean +/- SD, n = 10). The chemical assay of DG using total ferulic acid content would be a better choice to assess the herbal quality and was recommended. (c) 2005 Elsevier B.V. All rights reserved.
机译：当归（Dol）加工后的根当归（DG）的活性与阿魏酸的含量有关，但是阿魏酸在药用提取过程中的稳定性尚不清楚。使用多种提取溶剂评估了DG提取物中阿魏酸和阿魏针叶树素的稳定性。这些包括水中的甲醇，水，甲酸，1M盐酸水溶液和2％碳酸氢钠（NaHCO3）的各种组合和比例。在中性，强酸性和碱性溶剂中，针叶树阿魏酸针叶树酯易于水解成阿魏酸，在这种溶剂中，热量和水会促进这种水解。但是，在弱有机酸中相对抗水解。在稳定性评估的基础上，提出并定义了两个新术语：游离阿魏酸和总阿魏酸。游离阿魏酸是指草药中阿魏酸的天然含量。总阿魏酸是指游离阿魏酸的总和加上相关水解成分的量。同时，开发了一种高效液相色谱（HPLC）方法，以甲醇-甲酸（95：5）和甲醇-2％的NaHCO3水溶液（95：5）作为萃取液来测定DG中的游离阿魏酸和总阿魏酸。分别溶剂。研究了十个DG样品中游离和总阿魏酸的含量。结果表明，游离阿魏酸的含量变化幅度较大，总阿魏酸与游离阿魏酸之比为4.07 +/- 2.73（平均值+/- SD，n = 10）。建议使用总阿魏酸含量的DG进行化学分析，是评估草药质量的更好选择。 （c）2005 Elsevier B.V.保留所有权利。

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