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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >DETERMINATION OF INORGANIC CATIONS IN FERMENTATION AND CELL CULTURE MEDIA USING CATION-EXCHANGE LIQUID CHROMATOGRAPHY AND CONDUCTIVITY DETECTION
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DETERMINATION OF INORGANIC CATIONS IN FERMENTATION AND CELL CULTURE MEDIA USING CATION-EXCHANGE LIQUID CHROMATOGRAPHY AND CONDUCTIVITY DETECTION

机译：阳离子交换色谱法和电导率检测法测定发酵和细胞培养基中的无机阳离子

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摘要

Reliable methods to quantitate inorganic cations in biological culture media are often helpful for medium optimization and comprehensive process monitoring and control. Analysis of inorganic cations found in culture media was accomplished by ion chromatography for several types of microbial fermentations as well as mammalian and insect cell culture samples. An isocratic method consisting of a Dionex IonPac CS12 analytical column and 4 mM methanesulfonic acid mobile phase was used with suppressed conductivity detection. Sample preparation was a simple dilution of filtered broth with water. The analyses time was 20 min. This analysis accurately monitored eight inorganic cations, several of which are commonly present in fermentation media (Na+, NH4+ K+, Mg2+, Ca2+).
机译：定量生物培养基中无机阳离子的可靠方法通常有助于培养基优化和全面的过程监控。通过离子色谱对几种类型的微生物发酵以及哺乳动物和昆虫细胞培养物样品进行分析，可对培养基中发现的无机阳离子进行分析。使用由Dionex IonPac CS12分析柱和4 mM甲磺酸流动相组成的等度方法，抑制了电导率检测。样品制备是将滤过的肉汤用水简单稀释。分析时间为20分钟。该分析可准确监测八种无机阳离子，其中几种通常存在于发酵培养基中（Na +，NH4 + K +，Mg2 +，Ca2 +）。

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