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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Separation of solanesol in tobacco leaves extract by slow rotary counter-current chromatography using a novel non-aqueous two-phase solvent system
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Separation of solanesol in tobacco leaves extract by slow rotary counter-current chromatography using a novel non-aqueous two-phase solvent system

机译:新型非水两相体系慢旋转逆流色谱法分离烟叶提取物中的茄尼醇

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摘要

Non-aqueous solvent system composed of sunflower oil–ethanol was found to be suitable for the separation of lower polarity components. Employing this solvent system, 3 g of tobacco leaves extract containing 15% solanesol was separated using a slow rotary counter-current chromatograph equipped with an 1100 mL column made of 5.7 mm I.D. convoluted PTFE tube. The separation yielded 1.5 g of yellow oily product in which solanesol occupied 26.8% determined by HPLC. The tobacco tar and other main impurities in the tobacco leaves extract were removed after slow rotary counter-current chromatographic separation. The separation processing may be developed as a technique for producing a solanesol product with food grade.
机译:发现由葵花籽油-乙醇组成的非水溶剂体系适用于分离极性较低的组分。采用这种溶剂体系,使用装有5.7mm I.D的1100mL柱的慢旋转逆流色谱仪分离3g含15%茄尼醇的烟叶提取物。弯曲的PTFE管。分离得到1.5g黄色油状产物,其中通过HPLC测定,茄尼醇占26.8%。缓慢旋转逆流色谱分离后,去除烟叶提取物中的焦油和其他主要杂质。分离工艺可以发展为生产食品级茄尼醇产品的技术。

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