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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >High capacity organic monoliths for the simultaneous application to biopolymer chromatography and the separation of small molecules
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High capacity organic monoliths for the simultaneous application to biopolymer chromatography and the separation of small molecules

机译：高容量有机整体物，可同时应用于生物聚合物色谱法和小分子分离

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摘要

A method for controlling the mesoporous structure of monolithic organic copolymers is p resented by systematic variation in polymerisation time, employing poly(p-methylstyrene-co-1,2-(P-vinylphenyl)ethane) (MS/BVPE) as a representative styrene system. Decreasing the time of polymerisation introduces a considerable fraction of mesopores (up to 20% of the total pore Volume), while keeping the support permeability reasonable high (similar to 1.3 x 10(-14) m(2)). Monolith structures, prepared in such a manner, enable efficient (typically around 70,000 plates/m) and fast separation of low-molecular-weight compounds, whereas their performance towards biopolymers is comparable to column supports, fabricated according to typically used protocols (polymerisation time >12 h and thus monomer conversion >98%). The polymerisation time is hence a valuable tool to tailor the fraction of support flow-channels, macropores as well as mesopores, which is shown dramatically to influence the chromatographic separation characteristics of the respective column. This way, the preferred applicability of organic (styrene) monolithic copolymers can be extended to the separation of small molecules beyond biopolymer chromatography.
机译：以聚（对甲基苯乙烯-co-1,2-（对乙烯基苯基）乙烷）（MS / BVPE）为代表的苯乙烯，通过系统地改变聚合时间，提出了一种控制整体有机共聚物介孔结构的方法。系统。缩短聚合时间会引入相当一部分中孔（最多占总孔体积的20％），同时保持较高的支撑渗透率（类似于1.3 x 10（-14）m（2））。以这种方式制备的整体结构可实现高效（通常约为70,000板/米）和低分子量化合物的快速分离，而其对生物聚合物的性能可与根据通常使用的方案（聚合时间）制备的柱载体媲美。 > 12小时，因此单体转化率> 98％）。因此，聚合时间是调整载体流动通道，大孔以及中孔的比例的宝贵工具，其显示出显着影响相应色谱柱的色谱分离特性。这样，有机（苯乙烯）整体式共聚物的优选适用性可以扩展到生物聚合物色谱法以外的小分子分离。

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