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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Application of poly(dimethylsiloxane)/glass microchip for fast electrophoretic separation of serum small, dense low-density lipoprotein
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Application of poly(dimethylsiloxane)/glass microchip for fast electrophoretic separation of serum small, dense low-density lipoprotein

机译：聚二甲基硅氧烷/玻璃微芯片在快速电泳分离血清小而致密的低密度脂蛋白中的应用

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摘要

Due to the mounting evidence of altered low-density lipoprotein (LDL) size in several disease states, there has been an increasing interest in developing new analytical methods for small, dense low-density lipoprotein (sdLDL) for diagnosis. The present report demonstrates that sdLDL analysis can be performed in a poly(dimethylsiloxane) (PDMS/glass) microchannel. n-Dodecyl beta-D-maltoside (DDM) was utilized to alter channel surface to make it become hydrophilic and nonionic, thus reducing the interaction between the protein and the surface. Moreover, hydroxypropylcellulose (HPC) was added into the running buffer to suppress the adsorption of analytes and also to serve as a sieving matrix. Under optimal conditions, two baseline separations of lipoproteins including high-density lipoprotein (HDL), sdLDL, and ILDL were achieved with different selectivity. LDL particles shown on the electropherogram were also identified by several procedures. This method affords high separation speed and high reproducibility. The intraassay and interassay RSDs of lipoprotein migration times were in the range of 2.01-2.45%. The variation of serum sdLDL of a patient between prior treatment and post-treatment was assessed by this method. This system has the potential for rapid and sensitive detection of different LDL forms, and thus will be applicable to clinical diagnosis.
机译：由于越来越多的证据表明在几种疾病状态下低密度脂蛋白（LDL）大小发生了变化，因此人们越来越有兴趣开发新的分析方法用于诊断小而致密的低密度脂蛋白（sdLDL）。本报告表明sdLDL分析可以在聚（二甲基硅氧烷）（PDMS /玻璃）微通道中进行。正十二烷基β-D-麦芽糖苷（DDM）用于改变通道表面，使其变为亲水性和非离子性，从而减少了蛋白质与表面之间的相互作用。此外，将羟丙基纤维素（HPC）添加到运行缓冲液中，以抑制分析物的吸附，并用作筛分基质。在最佳条件下，脂蛋白的两个基线分离（包括高密度脂蛋白（HDL），sdLDL和ILDL）具有不同的选择性。电泳图上显示的LDL颗粒也可以通过几种方法进行鉴定。该方法提供了高分离速度和高再现性。脂蛋白迁移时间的测定内和测定间RSD在2.01-2.45％的范围内。通过这种方法评估了患者在治疗前和治疗后血清sdLDL的变化。该系统具有快速，灵敏地检测不同LDL形式的潜力，因此可应用于临床诊断。

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