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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >USE OF ION CHROMATOGRAPHY FOR MONITORING MICROBIAL SPOILAGE IN THE FRUIT JUICE INDUSTRY
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USE OF ION CHROMATOGRAPHY FOR MONITORING MICROBIAL SPOILAGE IN THE FRUIT JUICE INDUSTRY

机译：离子色谱技术在果汁工业中监测微生物的产生

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摘要

Fruit juices and purees are defined as fermentable, but unfermented, products obtained by mechanical processing of fresh fruits. The presence of undesired metabolites derived from microbial growth can arise from the use of unsuitable fruit or from defects in the production line or subsequent contamination. This involves a loss in the overall quality that cannot be resolved by thermal treatment following the start of fermentation. With these considerations, to ether with microbiological control, the analysis of different metabolites, which can be considered as microbial growth markers, such as alcohols (i.e. ethanol, etc.), acids (i.e. acetic, fumaric, lactic, etc.) is fundamental in order to achieve a better evaluation of product quality. Enzymatic determination and other single-component analytical techniques are often used for the determination of these metabolites. When the microbial spoilage is not well known, this results in a long and cumbersome procedure. A versatile technique that is capable of determining many metabolites in one analysis could be helpful in improving routine quality control. For this purpose, an ion chromatographic technique, such as ion exclusion, for separation, and diode array spectrophotometry and conductivity, for detection, were evaluated. Both different industrial samples and inoculated samples were analyzed.
机译：果汁和果泥被定义为通过机械加工新鲜水果获得的可发酵但未经发酵的产品。微生物生长引起的不希望的代谢产物的存在可能是由于使用不合适的水果或生产线缺陷或随后的污染所致。这涉及整体质量的损失，发酵开始后无法通过热处理解决。考虑到这些因素，对具有微生物控制能力的醚而言，分析不同的代谢产物至关重要，这些代谢产物可以被视为微生物的生长标志，例如醇类（例如乙醇等），酸类（例如乙酸，富马酸，乳酸等）。为了达到更好的产品质量评价。酶法测定和其他单组分分析技术通常用于测定这些代谢产物。当微生物变质不为人所知时，这将导致漫长而繁琐的过程。一种能够在一项分析中确定许多代谢物的通用技术可能有助于改善常规质量控制。为此，评估了一种离子色谱技术，例如用于分离的离子排斥法，以及用于检测的二极管阵列分光光度法和电导率。分析了不同的工业样品和接种样品。

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