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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Simultaneous determination of seven bufadienolides in rat plasma after oral administration of Shexiang Baoxin Pill by liquid chromatography-electrospray ionization-tandem mass spectrometry: Application to a pharmacokinetic study
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Simultaneous determination of seven bufadienolides in rat plasma after oral administration of Shexiang Baoxin Pill by liquid chromatography-electrospray ionization-tandem mass spectrometry: Application to a pharmacokinetic study

机译：液相色谱-电喷雾电离-串联质谱法同时测定射香保心丸口服液后大鼠血浆中七种丁二烯内酯的含量：在药代动力学研究中的应用

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摘要

A liquid cnromatograpny-electrospray ionization-tandem mass spectrometry metnod was described for the simultaneous determination of resibufogenin, bufalin, gamabufotalin, telibufagin, arenobufagin, cinobufagin and bufotalin in rat plasma. Plasma samples were pretreated by liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out on an ACQUITY HSS T3 column with gradient elution using mobile phase consisting of acetonitrile-0.1% formic acid in water at a flow rate of 0.3 mL/min. All analytes showed good linearity over a wide concentration range (r>0.99). The lower limit of quantification was in the range of 0.5-10 ng/mL for seven bufadienolides. The mean recovery of the analytes ranged from 94.36 to 104.18%. The intra- and inter-day precisions were in the range of 1.74-13.78% and the accuracies were between 89.37 and 101.38%. The validated method was successfully applied to a pharmacokinetic (PK) study of the seven bufadienolides in rat plasma after oral administration of Shexiang Baoxin Pill (SBP). The selected PK marker compounds with typical efficacy/toxicity may provide a practical solution for marker compound selection and dosage design for the therapeutic drug monitoring and PK study of SBP in its clinical applications.
机译：描述了一种液体cnromatograpny-电喷雾电离串联质谱法，用于同时测定大鼠血浆中的瑞波夫菌素，bufalin，gamabufotalin，telibufagin，arenobufagin，cinobufagin和bufotalin。血浆样品通过乙酸乙酯液-液萃取进行预处理。色谱分离是在ACQUITY HSS T3色谱柱上进行的，使用流动相由乙腈-0.1％甲酸的水溶液以0.3 mL / min的流速进行梯度洗脱。所有分析物在宽浓度范围内均表现出良好的线性（r> 0.99）。七种丁二烯内酯的定量下限在0.5-10 ng / mL范围内。分析物的平均回收率在94.36至104.18％之间。日内和日间精度在1.74-13.78％范围内，精度在89.37和101.38％之间。经验证的方法已成功地应用于射香保心丸（SBP）口服后大鼠血浆中的七种丁二烯内酯的药代动力学（PK）研究。所选择的具有典型功效/毒性的PK标记物可为SBP在临床应用中的治疗性药物监测和PK研究提供标记化合物选择和剂量设计的实用解决方案。

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