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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >DETERMINATION OF MORPHINE AND RELATED ALKALOIDS IN CRUDE MORPHINE, POPPY STRAW AND OPIUM PREPARATIONS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY
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DETERMINATION OF MORPHINE AND RELATED ALKALOIDS IN CRUDE MORPHINE, POPPY STRAW AND OPIUM PREPARATIONS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

机译：胶束电动毛细管色谱法测定粗吗啡，鸦片草和鸦片制剂中的吗啡及相关碱

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摘要

A rapid method for the determination of morphine and related alkaloids in crude morphine, poppy straw and opium preparations by micellar electrokinetic capillary chromatography (MEKC) has been developed. Morphine, codeine, thebaine, oripavine, papaverine, narcotine, narceine, cryptopine and salutaridine were separated in less than 10 min using a 70 cm x 50 mu m I.D. uncoated fused-silica capillary column with a buffer consisting of 10% dimethylformamide, 90% 0.05 M cetyltrimethylammonium bromide, 0.01 M potassium dihydrogen orthophosphate, 0.01 M sodium tetraborate, pH 8.6. An applied voltage of -25 kV and a temperature of 28 degrees C gave the best separation of the alkaloids. Pholcodine was used as the internal standard. The compounds were detected by UV at 254 nm. The levels of morphine and related alkaloids determined by MEKC were in good agreement with those determined by high-performance liquid chromatography (HPLC). The coefficients of variation for area calculation (%C.V.) for multiple sample and standard injections by MEKC were slightly greater than for HPLC but were still acceptable (morphine content of poppy straw: %CV. MEKC 1.7%, %CV. HPLC 0.3%).
机译：建立了一种通过胶束电动毛细管色谱法（MEKC）快速测定粗吗啡，罂粟草和鸦片制品中吗啡及相关生物碱的方法。使用70 cm x 50μmI.D.在不到10分钟的时间内即可分离出吗啡，可待因，蒂巴因，奥利巴韦恩，罂粟碱，那可丁，水仙碱，隐型松果碱和salutaridine。未涂覆的熔融石英毛细管柱，其缓冲液由10％二甲基甲酰胺，90％0.05 M十六烷基三甲基溴化铵，0.01 M正磷酸二氢钾，0.01 M四硼酸钠（pH 8.6）组成。施加-25 kV的电压和28摄氏度的温度可使生物碱得到最佳分离。羟可可碱用作内标。通过254nm的UV检测化合物。通过MEKC测定的吗啡和相关生物碱的水平与通过高效液相色谱法（HPLC）测定的含量非常一致。 MEKC多次进样和标准进样的面积计算的变异系数（％CV）略大于HPLC，但仍然可以接受（罂粟草的吗啡含量：％CV。MEKC 1.7％，％CV。HPLC 0.3％） 。

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