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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Isolation of isochlorogenic acid isomers in flower buds of Lonicera japonica by high-speed counter-current chromatography and preparative high performance liquid chromatography
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Isolation of isochlorogenic acid isomers in flower buds of Lonicera japonica by high-speed counter-current chromatography and preparative high performance liquid chromatography

机译：高速逆流色谱和制备型高效液相色谱法分离忍冬花蕾中的异绿原酸异构体

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摘要

An efficient method was established by HSCCC combined with prep-HPLC for separating isochlorogenic acid isomers from flower buds of Lonicera japonica. The partially purified sample from the methanol extract of flower buds of L. japonica by silica gel column was separated by HSCCC to result in a fraction containing two isochlorogenic acid isomers. The fraction was further isolated by prep-HPLC to yield isochlorogenic acid A and isochlorogenic acid C with purities of 98% and 96%, and the total recoveries at 80.1% and 79.8%, respectively. The chemical structures of isochlorogenic acid isomers were confirmed by electrospray ionization mass spectrometry (ESI-MS) and H-1 nuclear magnetic resonance (NMR). (C) 2014 Elsevier B.V. All rights reserved.
机译：HSCCC结合制备型HPLC建立了一种有效的方法，从忍冬中分离出异绿原酸异构体。通过HSCCC从硅胶提取的粳稻花蕾甲醇提取物中得到的部分纯化样品经HSCCC分离，得到含有两种异绿原酸异构体的馏分。通过制备型HPLC进一步分离级分，得到异绿原酸A和异绿原酸C，纯度为98％和96％，总回收率分别为80.1％和79.8％。通过电喷雾电离质谱（ESI-MS）和H-1核磁共振（NMR）证实了异绿原酸异构体的化学结构。 （C）2014 Elsevier B.V.保留所有权利。

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