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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Development and validation of an UFLC-MS/MS assay for the absolute quantitation of nine notoginsenosides in rat plasma: Application to the pharmacokinetic study of Panax Notoginseng Extract
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Development and validation of an UFLC-MS/MS assay for the absolute quantitation of nine notoginsenosides in rat plasma: Application to the pharmacokinetic study of Panax Notoginseng Extract

机译：UFLC-MS / MS测定和开发用于大鼠血浆中九种三七皂甙的绝对定量：在三七提取物的药代动力学研究中的应用

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摘要

Notoginsenosides, the main active gradients of Chinese traditional medicine Panax notoginseng, possesses a variety of biological activities including antioxidant property, anti-hyperglycemic, anti-obese, etc. However, pharmacokinetic evaluation for notoginsenosides is still a formidable task due to their low concentrations and complex components in vivo. The summation of this work generated a rapid and sensitive method for quantitative analysis of multi-notoginsenoside in rat plasma based on ultra fast liquid chromatographic-tandem mass spectrometric. After liquid-liquid extraction by n-butanol, notoginsenoside R1, Rg3, Rd, Rg2, Rb2, Rf, Rg1, Rb1 and Re were simultaneously monitored in negative ionization mode after separating on a Thermo ODS C-18 column (5 mm 50 mm x 2.1 mm) by a binary gradient elution, and all compounds were analyzed within 9 min. Multiple reaction monitoring (MRM) was performed as follows: R1 (m/z 967.7 --> 637.4), Rg3 (m/z 819.6 --> 621.4), Rd (m/z 981.6 --> 783.5), Rg2 (m/z 819.6 --> 475.4), Rb2 (m/z 1113.4 --> 783.4), Rf (m/z 835.6 --> 475.4), Rg1 (m/z 835.6 --> 637.6), Rb1 (m/z 1143.7 --> 945.6), Re (m/z 981.6 --> 637.4), internal standard (digoxin, m/z 815.5 --> 779.4). Validation parameters (linearity, sensitivity, intra-and inter-assay precision and accuracy, recovery and matrix effect) were within acceptable ranges and biological extracts were stable during the entire storing and preparing process. This UFLC-MS/MS approach was further validated by being applied to the pharmacokinetic study for P. Notoginseng extract in rats, and the pharmacokinetic parameters were calculated by Winolin software. Thus, the presently developed methodology was simple, robust, accurate, precise, and would be useful for the pharmacokinetic studies for all kinds of notoginsenosides and other herbal saponins. (C) 2015 Elsevier B.V. All rights reserved.
机译：三七皂苷是中药三七的主要活性梯度，具有多种生物活性，包括抗氧化，降血糖，抗肥胖等。然而，由于三七皂苷的浓度低，药理学含量高，其药代动力学评价仍是一项艰巨的任务。体内复杂的成分。这项工作的总结为基于超快速液相色谱-串联质谱的大鼠血浆中多种三七皂苷的定量分析提供了一种快速而灵敏的方法。用正丁醇进行液-液萃取后，在Thermo ODS C-18色谱柱（5 mm 50 mm x 2.1毫米）通过二元梯度洗脱，并在9分钟内分析了所有化合物。多反应监测（MRM）的执行方式如下：R1（m / z 967.7-> 637.4），Rg3（m / z 819.6-> 621.4），Rd（m / z 981.6-> 783.5），Rg2（m / z 819.6-> 475.4），Rb2（m / z 1113.4-> 783.4），Rf（m / z 835.6-> 475.4），Rg1（m / z 835.6-> 637.6），Rb1（m / z 1143.7-> 945.6），Re（m / z 981.6-> 637.4），内标（地高辛，m / z 815.5-> 779.4）。验证参数（线性，灵敏度，批内和批间精密度和准确性，回收率和基质效应）均在可接受的范围内，生物提取物在整个存储和制备过程中均稳定。将该UFLC-MS / MS方法应用于三七提取物在大鼠体内的药代动力学研究中进一步验证，并通过Winolin软件计算药代动力学参数。因此，目前开发的方法简单，可靠，准确，准确，对于各种三七皂苷和其他草药皂苷的药代动力学研究将是有用的。 （C）2015 Elsevier B.V.保留所有权利。

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