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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Isolation and purification of salvianolic acid A and salvianolic acid B from Salvia miltiorrhiza by high-speed counter-current chromatography and comparison of their antioxidant activity
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Isolation and purification of salvianolic acid A and salvianolic acid B from Salvia miltiorrhiza by high-speed counter-current chromatography and comparison of their antioxidant activity

机译：高速逆流色谱法分离丹参中丹酚酸A和丹酚酸B及其抗氧化活性比较

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摘要

Water-soluble salvianolic acid A (Sal A) and salvianolic acid B (Sal B) were successfully isolated and purified from the crude extract of Salvia miltiorrhiza by high-speed counter-current chromatography (HSCCC). The solvent system was n-hexane-ethyl acetate-methanol-water (3:6:6: 10, v/v/v/v). 4.27 mg of Sal A and 32.09 mg of Sal B were obtained from 260 mg of the crude sample. The purities of Sal A and Sal B were 96.67% and 97.43%, respectively. Their structures were identified by H-1 NMR and C-13 NMR. Antioxidant activities of Sal A and Sal B were also evaluated and compared by the methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS(center dot+)) radical cation decolourisation assay. Both Sal A and Sal B showed high radical scavenging activities with their EC50 values being 1.43 +/- 0.09 and 1.81 +/- 0.01 mu g/ml in DPPH radical method. The ABTS results showed that Sal A and Sal B exhibited high total antioxidant activities, their EC50 values were 1.35 +/- 0.00 and 1.43 +/- 0.01 mu g/ml, respectively. Crown Copyright
机译：通过高速逆流色谱法（HSCCC）从丹参的粗提物中成功分离并纯化了水溶性丹酚酸A（Sal A）和丹酚酸B（Sal B）。溶剂体系是正己烷-乙酸乙酯-甲醇-水（3∶6∶6∶10，v / v / v / v）。从260 mg粗样品中获得4.27 mg的Sal A和32.09 mg的SalB。 Sal A和Sal B的纯度分别为96.67％和97.43％。通过H-1 NMR和C-13 NMR鉴定它们的结构。还通过1,1-二苯基-2-吡啶并肼基（DPPH）自由基清除法和2,2-叠氮基-双-（3-乙基苯并噻唑啉-6-磺酸）方法评估和比较了Sal A和Sal B的抗氧化活性。 （ABTS（中心点+））自由基阳离子脱色试验。在DPPH自由基法中，Sal A和Sal B均表现出较高的自由基清除活性，其EC50值分别为1.43 +/- 0.09和1.81 +/- 0.01μg / ml。结果表明，Sal A和Sal B具有较高的总抗氧化活性，其EC50值分别为1.35 +/- 0.00和1.43 +/- 0.01μg / ml。

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