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首页> 外文期刊>Journal of consumer protection and food safety >Preparative purification of rebaudioside A from aqueous extracts using chromatography: a process idea.
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Preparative purification of rebaudioside A from aqueous extracts using chromatography: a process idea.

机译：使用色谱法从水性提取物中制备莱鲍迪甙A的制备纯化：一种工艺思想。

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摘要

An innovative chromatographic process idea for preparative purification of rebaudioside A from aqueous extracts of Stevia rebaudiana Bertoni leaves is developed. The process can handle high concentrated aqueous extracts, needs less equipment and has a lower solvent consumption compared to the state-of-the-art purification process of rebaudioside A. The innovative process consists of a cascade of three preparative chromatographic columns. The first column separates steviol glycosides from the plant matrix using water as eluent in reversed phase mode. The fraction of steviol glycosides is then concentrated on a second column which works as a capture column with a more non-polar adsorbent. The last column separates rebaudioside A from steviol glycoside under normal phase conditions using a mixture of acetonitrile and water as eluent. With a total yield of 97.5 +or- 1.5% and a purity of 97.1 +or- 1.5% the process fulfills the legal requirements for the purity of rebaudioside A. copyright Bundesamt fuer Verbraucherschutz und Lebensmittelsicherheit (BVL) 2012.
机译：从甜叶菊甜叶菊的水提取物中制备纯化莱鲍迪甙A的创新色谱方法构想得到了发展。与莱鲍迪甙A的最新纯化方法相比，该方法可以处理高浓度的水提取物，需要的设备更少，溶剂消耗也更低。该创新方法由三级制备色谱柱组成。第一列使用水作为反相模式的洗脱液从植物基质中分离甜菊醇糖苷。然后将部分甜菊醇糖苷浓缩在第二个柱子上，该柱子用作具有更多非极性吸附剂的捕获柱。最后一列使用乙腈和水的混合物作为洗脱液，在正相条件下将莱鲍迪甙A与甜菊糖苷分离。该方法的总收率为97.5±1.5％，纯度为97.1±1.5％，符合莱鲍迪甙A纯度的法律要求。版权所有2012，德国联邦广播电视大学（BVL）。

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