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首页> 外文期刊>Journal of Controlled Release: Official Journal of the Controlled Release Society >Protein bioactivity and polymer orientation is affected by stabilizer incorporation for double-walled microspheres
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Protein bioactivity and polymer orientation is affected by stabilizer incorporation for double-walled microspheres

机译：双层微球稳定剂的加入会影响蛋白质的生物活性和聚合物的取向

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Double-walled microspheres present an improved drug delivery technique for sustained release of encapsulated substrates. In this study, the release kinetics and biological activity of lysozyme was analyzed from microspheres comprised of poly (lactic-co-glycolic acid) (PLGA) and poly(L-lactide) (PLLA). In addition, coencapsulation of the anionic surfactant, docusate sodium salt (AOT), was investigated as a method of decreasing protein denaturation during microsphere fabrication. Herein, we show that through the inclusion of AOT, the capacity for two chemically similar polymers to phase separate and form double-walled (DW) microspheres is impaired leading to unique protein release kinetics. Additionally, we present the time period over which our released enzyme, lysozyme, remains biologically active. The consequences of AOT on protein bioactivity are also assessed and provide strong implications for the importance of appropriate stabilizer analysis in future studies involving drug co-encapsulates in polymer based microsphere systems.
机译：双壁微球呈现出用于持续释放包封的底物的改进的药物递送技术。在这项研究中，从由聚乳酸-乙醇酸（PLGA）和聚L-丙交酯（PLLA）组成的微球中分析了溶菌酶的释放动力学和生物学活性。此外，阴离子表面活性剂多库酯钠盐（AOT）的共包封被研究为减少微球制造过程中蛋白质变性的方法。在这里，我们表明通过包含AOT，两种化学相似的聚合物相分离并形成双壁（DW）微球的能力受到损害，从而导致独特的蛋白质释放动力学。此外，我们介绍了我们释放的酶溶菌酶保持生物学活性的时间段。还评估了AOT对蛋白质生物活性的影响，并对在涉及基于聚合物的微球系统中的药物共包封的未来研究中适当的稳定剂分析的重要性提供了重要的启示。

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