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首页> 外文期刊>Journal of Controlled Release: Official Journal of the Controlled Release Society >Porous nano-HA/collagen/PLLA scaffold containing chitosan microspheres for controlled delivery of synthetic peptide derived from BMP-2
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Porous nano-HA/collagen/PLLA scaffold containing chitosan microspheres for controlled delivery of synthetic peptide derived from BMP-2

机译：包含壳聚糖微球的多孔纳米HA /胶原蛋白/ PLLA支架，用于受控递送BMP-2衍生的合成肽

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摘要

It is advantageous to incorporate controlled growth factor delivery into tissue engineering strategies. The purpose of the present study was to develop a novel tissue engineering scaffold with the capability of controlled releasing BMP-2-derived synthetic peptide. Porous nano-hydroxyapatite/collagen/poly(L-lactic acid)/chitosan microspheres (nHAC/PLLA/CMs) composite scaffolds containing different quantities of chitosan microspheres (CMs) were prepared by a thermally induced phase separation method. Dioxane was used as the solvent for PLLA. Introduction of less than 30% of CMs (on PLLA weight basis) did not remarkably affect the morphology and porosity of the nHAC/PLLA/CMs scaffolds. However, as the microspheres contents increased to 50%, the porosity of the composite decreased rapidly. The compressive modulus of the composite scaffolds increased from 15.4 to 25.5 MPa, while the compressive strength increased from 1.42 to 1.63 MPa as the microspheres contents increased from 0% to 50%. The hydrolytic degradation and synthetic peptide release kinetics in vitro were investigated by incubation in phosphate buffered saline solution (pH 7.4). The results indicated that the degradation rate of the scaffolds was increased with the enhancement of CMs dosage. The synthetic peptide was released in a temporally controlled manner, depending on the degradation of both incorporated chitosan microspheres and PLLA matrix. In vitro bioactivity assay revealed that the encapsulated synthetic peptide was biologically active as evidenced by stimulation of rabbit marrow mesenchymal stem cells (MSCs) alkaline phosphatase (ALP) activity. The successful microspheres-scaffold system offers a new delivery method of growth factors and a novel scaffold design for bone regeneration.
机译：将受控的生长因子递送结合到组织工程策略中是有利的。本研究的目的是开发一种新型的组织工程支架，该支架具有控制释放BMP-2衍生的合成肽的能力。通过热诱导相分离法制备了多孔纳米羟基磷灰石/胶原蛋白/聚（L-乳酸）/壳聚糖微球（nHAC / PLLA / CMs）复合支架。二恶烷用作PLLA的溶剂。引入少于30％的CM（以PLLA重量计）不会显着影响nHAC / PLLA / CMs支架的形态和孔隙率。但是，随着微球含量增加到50％，复合材料的孔隙率迅速下降。随着微球含量从0％增加到50％，复合支架的压缩模量从15.4 MPa增加到25.5 MPa，而压缩强度从1.42 MPa增加到1.63 MPa。通过在磷酸盐缓冲盐溶液（pH 7.4）中孵育来研究体外的水解降解和合成肽释放动力学。结果表明，随着CMs剂量的增加，支架的降解率增加。取决于掺入的壳聚糖微球和PLLA基质的降解，合成肽以时间控制的方式释放。体外生物活性测定表明，经刺激的兔骨髓间充质干细胞（MSCs）碱性磷酸酶（ALP）活性可证明所包封的合成肽具有生物活性。成功的微球支架系统提供了生长因子的新递送方法和用于骨再生的新颖支架设计。

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