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首页> 外文期刊>Journal of crop science and Biotechnology >Identification of the leaf rust resistance genes Lr9, Lr26, Lr28, Lr34, and Lr35 in a collection of Iranian wheat genotypes using STS and SCAR markers.
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Identification of the leaf rust resistance genes Lr9, Lr26, Lr28, Lr34, and Lr35 in a collection of Iranian wheat genotypes using STS and SCAR markers.

机译：使用STS和SCAR标记鉴定一组伊朗小麦基因型中的叶锈病抗性基因Lr9，Lr26，Lr28，Lr34和Lr35。

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Brown rust or leaf rust is one of the most important diseases of wheat occurring almost in all wheat-producing regions and reduces crop yield. In order to produce resistant cultivars, it is necessary to identify resistance genes in different germplasms and combine them in (a) suitable stock(s). To identify the presence of the leaf rust resistance genes using STS and SCAR markers, 83 Iranian wheat genotypes, Lr near-isogenic lines in Thatcher (positive controls), and the cultivar Thatcher (negative control) were used. After growing plants in the greenhouse, DNA was extracted by SDS method. Following that, polymerse chain reaction was performed for the markers of the resistance genes Lr9, Lr26, Lr28, Lr34, and Lr35 which amplified 1,100, 1,100, 378, 150, and 900 bp bands, respectively. Based on the results, the resistance genes Lr9 and Lr35 were only present in the positive controls. The resistance gene Lr26 was only detected in four cultivars; Arta, Pishtaz, Shiroodi, and Falat, and the gene Lr34 was present in six cultivars (Akbari, Bam, Tajan, Khazar 1, Sistan and Niknezhad). The Lr28 primer amplified a band of the same size in all genotypes even the negative control and therefore the presence/absence of this gene could not be validated. These results indicate the necessity for designing a specific primer for Lr28. In general, only the genes Lr26 and Lr34 were present in some genotypes. The genes Lr9 and Lr35 were not present in this collection and as based on rust surveys, no virulence has been detected for Lr9 and Lr28, so they could be transferred to suitable lines from donor sources.Digital Object Identifier http://dx.doi.org/10.1007/s12892-012-0035-9
机译：褐锈病或叶锈病是几乎在所有小麦产区都发生的最重要的小麦疾病之一，并降低了农作物的产量。为了产生抗性品种，有必要鉴定不同种质中的抗性基因，并将其组合到合适的种群中。为了使用STS和SCAR标记鉴定叶锈病抗性基因的存在，使用了83种伊朗小麦基因型，Thatcher中的Lr近等基因系（阳性对照）和Thatcher品种（阴性对照）。在温室中种植植物后，通过SDS方法提取DNA。之后，对分别扩增1100、1100、378、150和900 bp条带的抗性基因Lr9，Lr26，Lr28，Lr34和Lr35的标记物进行聚合物链反应。根据结果​​，抗性基因Lr9和Lr35仅存在于阳性对照中。仅在四个品种中检测到抗性基因Lr26。 Arta，Pishtaz，Shiroodi和Falat以及Lr34基因存在于六个品种（Akbari，Bam，Tajan，Khazar 1，Sistan和Niknezhad）中。 Lr28引物即使在阴性对照中也能在所有基因型中扩增出一条大小相同的条带，因此无法验证该基因的存在与否。这些结果表明设计针对Lr28的特异性引物的必要性。通常，在某些基因型中仅存在基因Lr26和Lr34。 Lr9和Lr35基因没有出现在这个收集物中，根据锈蚀调查，Lr9和Lr28没有检测到毒力，因此可以将它们从供体来源转移到合适的品系中。数字对象标识符http：//dx.doi .org / 10.1007 / s12892-012-0035-9

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