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首页> 外文期刊>Journal of crop science and Biotechnology >Detection of QTLs influencing panicle length, panicle grain number and panicle grain sterility in rice (Oryza sativa L.).
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Detection of QTLs influencing panicle length, panicle grain number and panicle grain sterility in rice (Oryza sativa L.).

机译：检测影响水稻穗长，穗粒数和穗粒无菌性的QTLs。

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摘要

The detection, characterization and use of quantitative traits loci, QTL, have significant potential to improve the efficiency of selective breeding of species. Therefore, a population with 59 advanced backcross lines (BC2F5), derived from a cross between IR64 and Tarome molaei. were studied in Tonekabon Rice Research Station of Iran in order to map QTLs for panicle length, number of grain per panicle, and panicle grain sterility in rice. The parental screening with 235 SSR markers in agarose and polyacrylamide gels revealed 114 markers with clear polymorphic bands. To search for QTLs associated with panicle length, number of grain per panicle, and panicle grain sterility, we constructed a genetic linkage map using 114 microsatellite markers. Positive and negative transgressive segregations were observed in BC2F5 lines for all traits. Using multiple interval mapping (MIM), a total of 20 putative QTLs were detected, of which eight were for panicle length, three for number of grains, and nine for panicle grain sterility. The maximum number of QTLs were mapped on chromosomes 1 and 2 with eight QTLs. These QTL markers could possible be utilized for marker-assisted selection.
机译：数量性状基因座QTL的检测，表征和使用具有提高物种选择性育种效率的巨大潜力。因此，从IR64与Tarome molaei杂交得到的种群有59条先进的回交系（BC 2 F 5 ）。在伊朗的Tonekabon水稻研究站进行了研究，以便绘制水稻穗长，每穗粒数和穗粒无菌性的QTL。在琼脂糖和聚丙烯酰胺凝胶中用235个SSR标记进行父母亲筛选，发现114个标记具有清晰的多态性条带。为了搜索与穗长，穗粒数和穗粒不育有关的QTL，我们使用114个微卫星标记构建了遗传连锁图。在所有性状的BC 2 F 5 品系中均观察到正向和负向的分离。使用多间隔映射（MIM），总共检测到20个假定的QTL，其中8个用于穗长，三个用于粒数，九个用于不育穗。 QTL的最大数量与八个QTL映射在1号和2号染色体上。这些QTL标记可以用于标记辅助选择。

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