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首页> 外文期刊>Journal of crop science and Biotechnology >Comparative potential of phenotypic, ISSR and SSR markers for characterization of sesame (Sesamum indicum L.) varieties from India.
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Comparative potential of phenotypic, ISSR and SSR markers for characterization of sesame (Sesamum indicum L.) varieties from India.

机译：表型，ISSR和SSR标记在表征印度芝麻（Sesamum indicum L.）品种方面的比较潜力。

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摘要

The assessment of genetic diversity is a primary concern of plant breeders and germplasm curators for which morphological traits are internationally accepted descriptors but are often associated with limitations due to G x E interactions. Phenotypic, ISSR, and SSR markers were employed to determine the genetic diversity and relationships among 20 commercially cultivated sesame genotypes representing different geographical regions of India. A narrow range of genetic dissimilarity (0.01-0.12) with mixed clustering was observed using morphological descriptors. A total 279 band positions with 57% polymorphism was amplified by ISSR primers, whereas 36 allelic positions with 73% polymorphism were produced by SSR primers with 100% reproducibility. The ISSR primers with high discrimination power were identified producing genotype-specific bands and distinguishing all the sesame genotypes used in this study. A poor correlation and lack of perfect congruence between phenotypes and marker-based analysis was indicated by the UPGMA dendrogram and PCA analysis. Our results suggest that SSR and ISSR markers provide a powerful tool for genotype identification and establishment of genetic relationships among sesame genotypes. We recommend the use of molecular markers for testing, required for obtaining intellectual property rights, and also for the analysis of genotypic diversity among sesame varieties.
机译：遗传多样性的评估是植物育种者和种质管理者的主要关注点，其形态特征是国际公认的描述词，但由于G x E相互作用而常常与局限性相关。使用表型，ISSR和SSR标记来确定代表印度不同地理区域的20种商业种植芝麻基因型之间的遗传多样性和相关性。使用形态学描述符观察到混合聚类的窄范围遗传差异（0.01-0.12）。通过ISSR引物扩增了279个多态性为57％的条带位置，而通过SSR引物产生了具有73％多态性的36个等位基因，其重复性为100％。鉴定出具有高判别力的ISSR引物，可产生基因型特异性条带，并区分本研究中使用的所有芝麻基因型。 UPGMA树状图和PCA分析表明，表型与基于标志物的分析之间相关性较差且缺乏完美的一致性。我们的结果表明，SSR和ISSR标记为基因型鉴定和芝麻基因型间遗传关系的建立提供了有力的工具。我们建议使用分子标记物进行测试，这是获得知识产权所必需的，还应用于分析芝麻品种之间的基因型多样性。

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