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首页> 外文期刊>Journal of cutaneous pathology >The role of RhoC in growth and metastatic capacity of melanoma.
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The role of RhoC in growth and metastatic capacity of melanoma.

机译：RhoC在黑色素瘤生长和转移能力中的作用。

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BACKGROUND: RhoC overexpression in tumor cells promotes invasive and metastatic behavior. RhoC expression levels have been correlated with tumor progression and metastasis in multiple human cancers. In melanoma, RhoC is upregulated in highly metastatic tumors. Induced expression in melanoma cell lines resulted in invasion and metastasis, whereas inhibition of RhoC reversed the metastatic phenotype both in vitro and in vivo. METHODS: RhoC mRNA and protein expression in two human melanoma cell lines (DX3aza and MeWo) and pooled primary melanocytes were investigated by means of real-time quantitative polymerase chain reaction and western blotting. RhoC protein expression was evaluated in 123 primary cutaneous melanoma samples by the use of immunohistochemistry and correlated with known prognostic features. RESULTS: RhoC upregulation was observed in the highly metastatic DX3aza cell line, whereas in MeWo, only low expression levels could be detected. RhoC expression in primary cutaneous melanoma was strongly associated with thicker and ulcerated tumors. RhoC expression was associated with the presence of lymphatic metastases at the time of diagnosis and shorter disease-free and overall survival rates, without being an independent predictor. CONCLUSION: These results further support a role for RhoC in growth and metastasis of melanoma.
机译：背景：RhoC在肿瘤细胞中的过度表达促进侵袭和转移行为。 RhoC表达水平已与多种人类癌症中的肿瘤进展和转移相关。在黑色素瘤中，RhoC在高度转移性肿瘤中上调。黑色素瘤细胞系中诱导的表达导致侵袭和转移，而对RhoC的抑制则在体外和体内均逆转了转移表型。方法：通过实时定量聚合酶链反应和蛋白质印迹法研究了两种人黑素瘤细胞系DX3aza和MeWo中RhoC的mRNA和蛋白表达，以及合并的原代黑素细胞。 RhoC蛋白表达通过免疫组织化学方法在123例原发性皮肤黑色素瘤样品中进行了评估，并与已知的预后特征相关。结果：在高度转移性的DX3aza细胞系中观察到RhoC上调，而在MeWo中，只能检测到低表达水平。 RhoC在原发性皮肤黑色素瘤中的表达与较厚且溃疡的肿瘤密切相关。 RhoC的表达与诊断时淋巴结转移的存在，无疾病和总体生存期较短有关，而没有独立的预测因素。结论：这些结果进一步支持RhoC在黑色素瘤的生长和转移中的作用。

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