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首页> 外文期刊>Journal of cutaneous pathology >Expression patterns of MITF during human cutaneous embryogenesis: evidence for bulge epithelial expression and persistence of dermal melanoblasts.
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Expression patterns of MITF during human cutaneous embryogenesis: evidence for bulge epithelial expression and persistence of dermal melanoblasts.

机译：人皮肤胚胎发生过程中MITF的表达模式：鼓膜上皮表达和皮肤黑素细胞持续存在的证据。

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The mechanisms whereby melanocytes populate the epidermis and developing hair follicles during embryogenesis are incompletely understood. Recent evidence implicates an intermediate mesenchymal stage in this evolutionary process in which HMB-45-positive melanocyte precursors ('melanoblasts') exist both in intradermal as well as intraepithelial and intrafollicular compartments. The melanocyte master transcriptional regulator, microphthalmia transcription factor (MITF), identifies mature melanocytes as well as melanocyte precursor stem cells that reside in the bulge region of the hair follicle. Methods: To better define the use of MITF expression in the evaluation of melanocyte ontogeny, human embryonic and fetal skin samples (n = 28) at 6-24 weeks gestation were studied immunohistochemically for expression of MITF and Mart-1. Adjacent step sections were evaluated to correlate staining patterns with cell localization in the intraepidermal, intrafollicular and intradermal compartments. Results: At 6-8 weeks, MITF and Mart-1-positive cells were primarily intradermal with only rare positive cells in the epidermis. By 12-13 weeks, most of these cells had migrated into the epidermis, predominantly the suprabasal layers. Between 15-17 weeks, these cells localized to the basal layer and colonized developing hair follicles. Rare intradermal MITF and Mart-1 positive cells were found as late as week 20. At 18-24 weeks, MITF and Mart-1 positive cells were identified in the outer root sheath, bulge, and follicular bulge epithelium, in addition to the epidermis. Unexpectedly, weak but diffuse nuclear MITF expression was also present in the keratinocytes of the bulge area. Conclusions: The in situ migratory fate of MITF/Mart-1-expressing cells in fetal skin involves a well-defined progression from intradermal to intraepidermal to intrafollicular localization. Occasional intradermal melanocytes may persist after the intraepithelial stages are completed, a finding of potential significance to melanocytic proliferations that may arise de novo within the dermis. Because MITF may play a role in stem cell maintenance, the presence of MITF in bulge epithelial cells suggests that it may be a novel marker for follicular stem cells of both epithelial and melanocytic lineage.
机译：黑色素细胞在胚发生过程中填充表皮和发育中的毛囊的机制尚不完全清楚。最近的证据表明，在该进化过程中处于中间间充质阶段，其中HMB-45阳性黑素细胞前体（“黑素细胞”）同时存在于皮内以及上皮内和卵​​泡内。黑色素细胞主转录调节剂小眼症转录因子（MITF）可以识别成熟的黑色素细胞以及位于毛囊隆起区域的黑色素细胞前体干细胞。方法：为了更好地定义MITF表达在评估黑色素细胞个体发育中的用途，对人类胚胎和胎儿皮肤样本（n = 28）在妊娠6-24周时进行了免疫组织化学研究，以研究MITF和Mart-1的表达。评估相邻的台阶切片以使染色模式与表皮内，卵泡内和皮内隔室中的细胞定位相关。结果：在6-8周时，MITF和Mart-1阳性细胞主要在皮内，表皮中只有罕见的阳性细胞。到12-13周时，大多数这些细胞已迁移到表皮中，主要是上基底层。在15-17周之间，这些细胞定位在基底层并定居在发育的毛囊中。直到第20周才发现罕见的皮内MITF和Mart-1阳性细胞。在18-24周时，除表皮外，在根部外皮，隆起和滤泡隆起上皮中还发现了MITF和Mart-1阳性细胞。 。出乎意料的是，在凸起区域的角质形成细胞中也存在弱而弥漫的核MITF表达。结论：胎儿皮肤中表达MITF / Mart-1的细胞的原位迁移命运涉及从皮内定位到表皮内定位到卵泡内定位的明确过程。上皮内阶段完成后，偶尔的皮内黑素细胞可能会持续存在，这一发现对于可能在真皮内新生的黑素细胞增殖具有潜在意义。因为MITF可能在干细胞维持中发挥作用，所以膨胀的上皮细胞中MITF的存在表明它可能是上皮和黑素细胞谱系的滤泡干细胞的新标记。

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