...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of drug targeting >Anionic LPD complexes for gene delivery to macrophage: preparation, characterization and transfection in vitro.
【24h】

Anionic LPD complexes for gene delivery to macrophage: preparation, characterization and transfection in vitro.

机译：阴离子LPD复合物，用于基因传递至巨噬细胞：体外制备，表征和转染。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the present study, anionic lipid/peptide/DNA (LPD) complexes consisting of pH-sensitive liposome and protamine were introduced as the carriers targeting RAW 264.7 cell line, which had been reported to be difficult for transfection. The LPD complexes were physically characterized. The pH sensitivities and sizes of liposomes were investigated. The zeta potentials of LPD complexes altered significantly with the addition of protamine sulfate and anionic liposomes. It was demonstrated that the carriers produced an increase in the stability of plasmid DNA against DNase I. The TEM showed that the size distribution of LPD complexes was irregular. In the in vitro transfection, the efficiency of LPD complexes was higher than that of Lipofectamine 2000 and protamine/DNA complexes, but lower than that of electroporation. A possible mechanism for the internalization of plasmid DNA mediated by the anionic LPD complexes was also proposed. With a high safety certificated by MTT assay, LPD complexes prepared in this study might be potentially employed as a macrophage gene therapy.
机译：在本研究中，由pH敏感脂质体和鱼精蛋白组成的阴离子脂质/肽/ DNA（LPD）复合物作为靶向RAW 264.7细胞系的载体被引入，据报道这是很难转染的。 LPD复合物进行了物理表征。研究了脂质体的pH敏感性和大小。添加硫酸鱼精蛋白和阴离子脂质体后，LPD复合物的ζ电位发生了显着变化。证明了载体增加了质粒DNA针对DNase I的稳定性。TEM显示LPD复合物的大小分布是不规则的。在体外转染中，LPD复合物的效率高于Lipofectamine 2000和鱼精蛋白/ DNA复合物，但低于电穿孔。还提出了由阴离子LPD复合物介导的质粒DNA内在化的可能机制。通过MTT分析证明具有高度安全性，本研究中制备的LPD复合物可能被潜在地用作巨噬细胞基因疗法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号