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首页> 外文期刊>Clinical chemistry and laboratory medicine: CCLM >Automated immunoassays for the autoantibodies to carbamylated or citrullinated telopeptides of type I and II collagens
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Automated immunoassays for the autoantibodies to carbamylated or citrullinated telopeptides of type I and II collagens

机译：针对I型和II型胶原的氨基甲酸酯化或瓜氨酸化的端肽自身抗体的自动免疫测定

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Background: The aim of the study was to describe automated immunoassays for autoantibodies to homocitrul-line or citrulline containing telopeptides of type I and II collagen in various disease categories in an early arthritis series.Methods: Serum samples were collected from 142 patients over 16 years of age with newly diagnosed inflammatory joint disease. All samples were analyzed with an automated inhibition chemiluminescence immunoassay (CLIA) using four different peptide pairs, each consisting of a biotinylated antigen and an inhibiting peptide. Assays were performed with an IDS-iSYS analyzer.Autoantibodies binding to homocitrulline and citrulline containing C-telopeptides of type I (HTELO-I, TELO-I) and type II collagens (HTELO-II, TELO-II) were analyzed. Results: The mean ratio of HTELO-I inhibition in sero-positive and seronegative rheumatoid arthritis (RA) was 3.07 (95% CI 1.41-11.60), p=0.003, and in seropositive and seronegative undifferentiated arthritis (UA) 4.90 (1.85-14.49), p<0.001. The respective mean ratios in seropositive and seronegative RA and UA were in TELO-I 8.72 (3.68-58.01), p<0.001 and 3.13 (1.49-6.16), p=0.008, in HTELO-II 7.57 (3.18-56.60), p<0.001 and 2.97 (1.23-6.69), p=0.037, and in TELO-II 3.01 (1.30-9.51), p=0.002 and 3.64 (1.86-7.65), p=0.008. In reactive arthritis, ankylosing spondyli-tis, psoriatic arthritis and unspecified spondyloarthritis the inhibition levels were similar to those observed in seronegative RA or UA.Conclusions: Autoantibodies binding to homocitrulline or citrulline containing telopeptides of type I and II collagen did not differ significantly. They were highest among patients with seropositive disease and they differentiated seropositive and seronegative arthritis.
机译：背景：这项研究的目的是描述针对早期关节炎系列疾病中各种疾病类别的针对含有同型瓜氨酸或瓜氨酸的I型和II型胶原端肽的自身抗体的自动免疫分析方法：方法：从16位患者中收集血清样本，历时16年新诊断的炎性关节疾病的年龄。使用四个不同的肽对，通过自动抑制化学发光免疫分析法（CLIA）对所有样品进行分析，每个肽对均由生物素化抗原和抑制性肽组成。使用IDS-iSYS分析仪进行分析，分析与包含I型C端肽（HTELO-I，TELO-I）和II型胶原蛋白（HTELO-II，TELO-II）的高瓜氨酸和瓜氨酸结合的自身抗体。结果：在血清阳性和血清阴性类风湿性关节炎（RA）中，HTELO-1抑制的平均比率为3.07（95％CI 1.41-11.60），p = 0.003，在血清阳性和血清阴性的未分化关节炎（UA）中为4.90（1.85- 14.49），p ＜0.001。血清阳性和血清阴性RA和UA的平均比率分别为TELO-1 8.72（3.68-58.01），p <0.001和3.13（1.49-6.16），p = 0.008，HTELO-II 7.57（3.18-56.60），p <0.001和2.97（1.23-6.69），p = 0.037，在TELO-II 3.01（1.30-9.51）中，p = 0.002和3.64（1.86-7.65），p = 0.008。在反应性关节炎，强直性脊柱炎，银屑病关节炎和未指明的脊椎关节炎中，抑制水平与血清​​阴性RA或UA中观察到的相似。结论：与同型瓜氨酸或瓜氨酸结合的I型和II型胶原端肽的自身抗体无显着差异。它们在血清反应阳性患者中最高，并且可以区分血清阳性和血清阴性关节炎。

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