Prediction of a coding sequence for a novel type II keratin from N-terminal sequences of mouse epidermal proteins site-specifically deiminated in embryonic development.

Senshu T; Ishida Yamamoto A; Takahashi H; Iizuka H

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Prediction of a coding sequence for a novel type II keratin from N-terminal sequences of mouse epidermal proteins site-specifically deiminated in embryonic development.

机译：从小鼠表皮蛋白在胚胎发育中位点特异性确定的N-末端序列预测新型II型角蛋白的编码序列。

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BACKGROUND: Epidermal keratinization involves various post-translational modifications including the deimination of arginine residues. Major deiminated proteins are derived from keratin K1. Two preferred deimination sites were identified in the V subdomain of mouse K1. An antibody against one of the deiminated peptide sequences (ACP) recognized deiminated mouse and human K1, and stained the cornified layers of human and infant mouse epidermis. ACP also stained the outermost layer of mouse embryonic epidermis. Western blotting revealed minor proteins showing strong ACP-positive signals in the mouse embryonic epidermal extract in which deiminated K1 derivatives were hardly detected. OBJECTIVE: To characterize ACP-positive proteins expressed in mouse embryonic epidermis. METHODS: ACP-positive proteins were isolated by preparative gel electrophoresis for N-terminal sequencing followed by blast searches for matching sequences in the protein and nucleotide database. RESULTS: We obtained N-terminal sequences of two ACP-positive proteins. A cDNA clone in the est_mouse database has an open reading frame for 202 amino acid residues containing both sequenced peptides. The deduced sequence shows typical features of the N-terminal portion of type II keratins. A virtually identical sequence to this reading frame is present in a genomic contig of chromosome 15 on which keratin type II genes are clustered. Sequential searches for overlapping cDNA clones in the est_mouse database along with similar searches in the genomic contig formulated a hypothetical cDNA sequence encoding a putative protein of 572 amino acid residues tentatively called K1-emb. CONCLUSION: We predicted a sequence of novel type II keratin site-specifically deiminated in embryonic mouse epidermis.

机译：背景：表皮角质化涉及各种翻译后修饰，包括精氨酸残基的确定。主要的脱脂蛋白来自角蛋白K1。在小鼠K1的V亚结构域中鉴定了两个优选的决定位点。针对其中一种脱肽肽序列（ACP）的抗体识别脱小鼠和人K1，并对人和婴儿小鼠表皮的角质层染色。 ACP还染色了小鼠胚胎表皮的最外层。蛋白质印迹显示，在小鼠胚胎表皮提取物中几乎没有蛋白显示出较强的ACP阳性信号，在该蛋白中几乎未检测到衍生的K1衍生物。目的：鉴定在小鼠胚胎表皮中表达的ACP阳性蛋白。方法：通过制备性凝胶电泳分离ACP阳性蛋白质，进行N端测序，然后快速搜索蛋白质和核苷酸数据库中的匹配序列。结果：我们获得了两个ACP阳性蛋白的N端序列。 est_mouse数据库中的cDNA克隆具有一个开放阅读框，可读取包含两个测序肽的202个氨基酸残基。推导的序列显示II型角蛋白N末端部分的典型特征。与该阅读框几乎相同的序列存在于第15号染色体的基因组重叠群中，II型角蛋白基因在其上聚集。在est_mouse数据库中对重叠cDNA克隆的顺序搜索以及在基因组重叠群中的相似搜索，制定了一个假设的cDNA序列，该序列编码假定为572个氨基酸残基的假定蛋白，暂称为K1-emb。结论：我们预测了在胚胎小鼠表皮中特异性定位的新型II型角蛋白序列。

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《Journal of dermatological science》 |2005年第1期|共8页
作者
Senshu T; Ishida Yamamoto A; Takahashi H; Iizuka H;
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正文语种 eng
中图分类皮肤病学与性病学;
关键词
Laboratory mice; Proteins; Cycloleucine; Keratin; 蛋白质类; 环亮氨酸; 角蛋白;

机译：Laboratory mice;Proteins;Cycloleucine;Keratin;蛋白质类;环亮氨酸;角蛋白;

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1. Prediction of a coding sequence for a novel type II keratin from N-terminal sequences of mouse epidermal proteins site-specifically deiminated in embryonic development. [J] . Senshu T, Ishida Yamamoto A, Takahashi H, Journal of dermatological science . 2005,第1期

机译：从小鼠表皮蛋白在胚胎发育中位点特异性确定的N-末端序列预测新型II型角蛋白的编码序列。
2. Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated librar [J] . Okazaki N, Kikuno R, Ohara R, DNA research: an international journal for rapid publication of reports on genes and genomes . 2003,第4期

机译：KIAA基因的小鼠同源物编码序列的预测：III。通过筛选随机从大小分离的文库中提取的cDNA克隆的末端序列来鉴定500个小鼠KIAA同源cDNA的完整核苷酸序列
3. Prediction of the Coding Sequences of Mouse Homologues of KIAA Gene: III. The Complete Nucleotide Sequences of 500 Mouse KIAA-homologous cDNAs Identified by Screening of Terminal Sequences of cDNA Clones Randomly Sampled from Size-fractionated Libraries [J] . Haruhiko Koseki, Hisashi Koga, Noriko Okazaki, DNA research: an international journal for rapid publication of reports on genes and genomes . 2003,第4期

机译：KIAA基因的小鼠同源物编码序列的预测：III。通过筛选大小分级文库的cDNA克隆末端序列来鉴定500个小鼠KIAA同源cDNA的完整核苷酸序列。
4. Prediction of Type III Secreted Effectors Based on Word Embeddings for Protein Sequences [C] . Xiaofeng Fu, Yiqun Xiao, Yang International Symposium on Bioinformatics Research and Applications . 2018

机译：基于蛋白质序列词嵌入的III型分泌效应的预测
5. Computational analysis of thermal denaturation differences and prediction of coding and non-coding eukaryotic DNA sequences. [D] . Long, Dang Duc. 2003

机译：热变性差异的计算分析以及编码和非编码真核DNA序列的预测。
6. The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60000 Da: analysis of sequence differences between type I and type II keratins. [O] . P M Steinert, D A Parry, E L Racoosin, 1984

机译：II型小鼠表皮角蛋白60000 Da的完整cDNA和推导的氨基酸序列：分析I型和II型角蛋白之间的序列差异。
7. The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 Da: analysis of sequence differences between type I and type II keratins. [O] . Steinert, P M, Parry, D A, Racoosin, E L, 1984

机译：II型小鼠表皮角蛋白60,000 Da的完整cDNA和推导的氨基酸序列：分析I型和II型角蛋白之间的序列差异。

Prediction of a coding sequence for a novel type II keratin from N-terminal sequences of mouse epidermal proteins site-specifically deiminated in embryonic development.

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