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首页> 外文期刊>Journal of dentistry >Effect of a CPP-ACP agent on the demineralization and remineralization of dentine in vitro.
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Effect of a CPP-ACP agent on the demineralization and remineralization of dentine in vitro.

机译：CPP-ACP试剂对体外牙本质脱矿质和再矿化的影响。

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OBJECTIVES: The aim of this study was to determine in vitro the effect of a commercial paste based on CPP-ACP complex on the demineralization of sound human dentine and on remineralization potential of artificial caries-like lesions formed on dentine surfaces. METHODS: Forty dentine specimens were prepared with hard tissue microtome. The specimens were divided in four groups the A, B, C and D (n=10). The specimen surfaces were subjected to surface analysis by Fourier transformance micro multiple internal reflectance infrared spectroscopy (micro MIR-FTIR). Tooth mousse was applied on surface specimens of A group, while no agent were applied on the specimens of B group. Afterwards, groups A, B, C and D were immersed in demineralization solution for 7 days. Afterwards, the surfaces were subjected to micro MIR-FTIR analysis and the mineral to matrix ratio was used to assess the extent of dentin demineralization (DM). Tooth mousse was applied on specimens of group C, while no agent was applied on specimens of group D. The groups C and D immersed in artificial saliva for 7 days and were subjected to analysis by micro MIR-FTIR and the mineral to matrix ratio was used to assess the extent of dentin remineralization (RM). RESULTS: Group A showed significant lower %DM in comparison to group B. Group C resulted in a significant higher %RM compared to group D. CONCLUSIONS: The presence of agent CPP-ACP on dentine surfaces provoked lower demineralization and higher remineralization in comparison with the dentine surfaces without agent.
机译：目的：本研究的目的是在体外确定基于CPP-ACP复合物的商业糊剂对人牙本质健全的脱矿质以及在牙本质表面形成的龋齿样病变的再矿化潜力的作用。方法：用硬组织切片机制备了40个牙质标本。将标本分为A，B，C和D四组（n = 10）。通过傅立叶变换显微多重内反射红外光谱仪（微型MIR-FTIR）对样品表面进行表面分析。在A组的表面标本上涂了摩丝，而B组的标本上没有涂任何剂。之后，将A，B，C和D组浸入脱盐溶液中7天。之后，对表面进行微MIR-FTIR分析，并使用矿物质与基质的比率来评估牙本质脱矿物质（DM）的程度。在C组的标本上施加了牙慕斯，而D组的标本上没有施加任何药剂。C组和D组在人工唾液中浸泡了7天，并通过微型MIR-FTIR进行了分析，矿物与基质的比用于评估牙本质再矿化（RM）的程度。结果：与B组相比，A组的％DM显着降低。C组与D组相比，％RM显着较高。结论：牙本质表面上存在CPP-ACP试剂的情况下，其脱矿质和较高的再矿化度牙本质表面无助剂。

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