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首页> 外文期刊>Journal of dermatological science >Nitroxides and a nitroxide-based UV filter have the potential to photoprotect UVA-irradiated human skin fibroblasts against oxidative damage.
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Nitroxides and a nitroxide-based UV filter have the potential to photoprotect UVA-irradiated human skin fibroblasts against oxidative damage.

机译：一氧化氮和基于一氧化氮的紫外线滤光片具有保护紫外线辐射的人皮肤成纤维细胞免受氧化损伤的潜力。

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BACKGROUND: Antioxidants are now being incorporated into sunscreens as additional topical measure for delaying the aging process and reducing photo-damage to skin induced by excessive UVA exposure. UVA radiation reaching the skin leads to the generation of ROS (reactive oxygen species) implicated in DNA damage and activation of matrix metalloproteinase-1 (MMP-1) responsible for collagen damage and photo-aging. Nitroxides are a class of compounds endowed with versatile antioxidant activity and recently, nitroxide-based UV filters in which a nitroxide moiety has been attached to the most popular UV filter present in sunscreens have been developed. OBJECTIVE: This study explores the potential photo-protective effects of these compounds on ROS production and induction of MMP-1 in cultured human dermal fibroblasts exposed to UVA. For comparison, vitamin E was also tested. METHODS: The effects were assessed by measuring intracellular ROS production using a ROS-index probe and MMP-1 mRNA expression levels using quantitative real-time PCR (qPCR). RESULTS: Exposure of fibroblasts to 18J/cm(2) UVA lead to a two-fold increase in ROS production which was reduced to non-irradiated control levels in the presence of 50muM nitroxide compounds and vitamin E. Under the same conditions, a ten-fold increase in MMP-1 mRNA expression levels was observed 24h post-UVA treatment which was significantly reduced by all nitroxide compounds but not vitamin E. CONCLUSION: The results of this study support the potential use of nitroxide compounds, including novel nitroxide-based UV filters, as a useful and alternative strategy for improving the efficacy of topical formulations against photo-aging and possibly photo-carcinogenesis.
机译：背景：抗氧化剂目前已被掺入防晒霜中，作为延缓衰老过程并减少因过度暴露于UVA引起的对皮肤的光损伤的附加局部措施。 UVA辐射到达皮肤会导致ROS（活性氧）的产生，这与DNA损伤和基质金属蛋白酶-1（MMP-1）的活化有关，后者负责胶原蛋白的损伤和光老化。氮氧化物是一类具有多种抗氧化剂活性的化合物，近来，已经开发出基于氮氧化物的紫外线过滤器，其中将氮氧化物部分连接到防晒霜中最流行的紫外线过滤器上。目的：本研究探讨了这些化合物对暴露于UVA的培养的人皮肤成纤维细胞中ROS产生和MMP-1诱导的潜在光保护作用。为了进行比较，还对维生素E进行了测试。方法：通过使用ROS指数探针测量细胞内ROS产生和使用定量实时PCR（qPCR）来测量MMP-1 mRNA表达水平来评估其作用。结果：成纤维细胞暴露于18J / cm（2）UVA导致ROS产量增加两倍，在50μM一氧化氮化合物和维生素E存在的情况下，ROS产量降低至非辐照对照水平。在相同条件下，十UVA处理后24h观察到MMP-1 mRNA表达水平增加了2倍，所有亚硝酸盐化合物均显着降低了MMP-1 mRNA表达水平，但维生素E却没有。紫外线过滤剂是提高局部制剂对抗光老化和可能光致癌作用的功效的一种有用的替代策略。

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