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首页> 外文期刊>Journal of dermatological science >Role of plasminogen activator inhibitor-2 (PAI-2) in keratinocyte differentiation.
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Role of plasminogen activator inhibitor-2 (PAI-2) in keratinocyte differentiation.

机译：纤溶酶原激活物抑制剂2（PAI-2）在角质形成细胞分化中的作用。

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BACKGROUND: Plasminogen activator inhibitor-2 (PAI-2) is an enzyme inhibitor which is involved in various biological processes including cell differentiation, tissue regrowth and regeneration. Although PAI-2 has been originally isolated as an extracellular inhibitor of urokinase plasminogen activator (uPA), recent studies indicate that PAI-2 has other intracellular effects in keratinocyte, such as the component of cornified envelope. OBJECTIVE: The aim of this study is to investigate the expression and functional role of PAI-2 during the keratinocyte differentiation. METHODS: We transduced keratinocytes with adenovirus harboring the expression cassette for PAI-2, then examined the effect on keratinocytes differentiation. RESULTS: When cultured epidermal keratinocytes were treated with 1.2 mM calcium, PAI-2 expression was increased time-dependently at both mRNA and protein levels. The calcium-induced PAI-2 expression was abolished by treatment with p38 MAPK inhibitor, while overexpression of MKK6 led to the increase of PAI-2 expression. When PAI-2 was overexpressed by adenoviral transduction, the expression of keratinocyte differentiation markers such as involucrin, keratin 10 and loricrin was markedly increased. Concomitantly, overexpression of PAI-2 resulted in the retardation of cell growth, with the increase of Rb and p53. CONCLUSION: These results suggest that PAI-2 has a role for promoting the differentiation of epidermal keratinocytes.
机译：背景：纤溶酶原激活物抑制剂2（PAI-2）是一种酶抑制剂，涉及多种生物学过程，包括细胞分化，组织再生和再生。尽管PAI-2最初是作为尿激酶纤溶酶原激活剂（uPA）的细胞外抑制剂而分离的，但最近的研究表明PAI-2在角质形成细胞中还具有其他细胞内作用，例如角质化包膜的成分。目的：研究PAI-2在角质形成细胞分化过程中的表达及其功能。方法：用带有PAI-2表达盒的腺病毒转导角质形成细胞，然后研究其对角质形成细胞分化的影响。结果：当用1.2 mM钙处理培养的表皮角质形成细胞时，PAI-2表达在mRNA和蛋白质水平上均呈时间依赖性增加。钙诱导的PAI-2表达通过用p38 MAPK抑制剂处理而被消除，而MKK6的过表达导致PAI-2表达的增加。当腺病毒转导过表达PAI-2时，角化细胞分化标志物（如总蛋白，角蛋白10和loricrin）的表达显着增加。伴随地，PAI-2的过表达导致细胞生长的延迟，Rb和p53的增加。结论：这些结果表明PAI-2具有促进表皮角质形成细胞分化的作用。

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