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首页> 外文期刊>Journal of dentistry >Dissolution of bio-active dentine matrix components by mineral trioxide aggregate.
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Dissolution of bio-active dentine matrix components by mineral trioxide aggregate.

机译：三氧化二无机矿物质溶解生物活性牙本质基质成分。

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OBJECTIVES: To analyze the soluble components of setting and set mineral trioxide aggregate (MTA), assess the abilities of two varieties of MTA and Ca(OH)(2) solutions to solubilise dentine matrix proteins (DMPs) and determine if these extracts contain signalling molecules important to pulpal repair and regeneration. METHODS: The metallic ion composition of solutions of white and grey MTA (pH 11.7), 0.02M Ca(OH)(2) (pH 11.9) and 10% EDTA (pH 7.2) was determined using atomic absorption spectroscopy. Extracellular dentine matrix components from powdered human dentine were extracted using all solutions over 14 days. Extracts were analysed for concentrations of non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs), and protein profiles were examined using 1D-polyacrylamide gel electrophoresis (1D-PAGE). ELISAs for TGF-beta1 and adrenomedullin (ADM) were also performed. RESULTS: Aluminium, calcium, potassium, and sodium ions were detected in both white and grey MTA solutions. MTA and Ca(OH)(2) solutions liberated similar amounts of GAGs and NCPs although yields were considerably lower than those obtained using the EDTA solution. 1D-PAGE analysis demonstrated differences in protein profiles solubilised from dentine for all solutions. All extracts contained TGF-beta1 and ADM, EDTA solution liberated significantly greater amounts of TGF-beta1, and Ca(OH)(2) and grey MTA solutions released more ADM. CONCLUSIONS: These data imply that when placed clinically soluble components of set and setting MTA may release dentine matrix components that potentially influence cellular events for dentine repair and regeneration.
机译：目的：为了分析凝固和凝固的三氧化二矿物聚集体（MTA）的可溶性成分，评估两种MTA和Ca（OH）（2）解决方案溶解牙本质基质蛋白（DMP）的能力，并确定这些提取物是否包含信号传导对牙髓修复和再生很重要的分子。方法：采用原子吸收光谱法测定了白色和灰色MTA（pH 11.7），0.02M Ca（OH）（2）（pH 11.9）和10％EDTA（pH 7.2）溶液的金属离子组成。在14天之内使用所有溶液从​​粉末状人类牙本质中提取细胞外牙本质基质成分。分析提取物中非胶原蛋白（NCPs）和糖胺聚糖（GAGs）的浓度，并使用1D-聚丙烯酰胺凝胶电泳（1D-PAGE）检查蛋白质谱。还对TGF-beta1和肾上腺髓质素（ADM）进行了ELISA。结果：在白色和灰色MTA溶液中均检测到铝，钙，钾和钠离子。 MTA和Ca（OH）（2）溶液释放出相似量的GAG和NCP，尽管产量大大低于使用EDTA溶液获得的产量。 1D-PAGE分析表明，对于所有溶液，从牙本质溶解的蛋白质谱都有差异。所有提取物均含有TGF-beta1和ADM，EDTA溶液释放出大量的TGF-beta1，Ca（OH）（2）和灰色MTA溶液释放出更多的ADM。结论：这些数据暗示当放置可凝固的和可凝固的MTA的临床上可溶成分时，可能释放出可能影响细胞事件的牙本质基质成分，从而影响牙本质的修复和再生。

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