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首页> 外文期刊>Journal of dentistry >In vitro microleakage of adhesive-sealed dentin with lactic acid and saliva exposure: a radio-isotope analysis.
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In vitro microleakage of adhesive-sealed dentin with lactic acid and saliva exposure: a radio-isotope analysis.

机译：粘合剂密封的牙本质在乳酸和唾液暴露下的体外微渗漏：放射性同位素分析。

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OBJECTIVES: The aim of this study was to evaluate the dentin-protective potential of two dentin-bonding systems (Syntac and Clearfil SE Bond) on artificial saliva and lactic acid exposure. METHODS: Radiochemical analysis in combination with polarization-microscopy was used in the current study. Different applications in MOD preparations in irradiated human molars were compared. Untreated cavities and cavities covered with flowable resin served as controls. Forty-eight specimens were immersed in artificial saliva (pH 7.5), 48 in lactic acid (pH 4). Activity of (32)P was measured between days 1 and 21 and loss of mineralized tissue was estimated. RESULTS: In lactic acid, cavities sealed with single bond, double bond or bond plus cavity sealer application resulted in significantly lower mineralized tissue loss compared to negative controls (p < 0.05). Upon both saliva and lactic acid exposure, bonding plus sealer application proved to be the most protective treatment (p < 0.05). CONCLUSIONS: Based on these in vitro findings it may be advisable to use a filled cavity sealer or a flowable resin to protect dentin from demineralization. The radiochemical analysis appeared to be valuable in evaluating the protective potential of bonding agents against dentin demineralization.
机译：目的：本研究的目的是评估两种牙本质粘合系统（Syntac和Clearfil SE Bond）对人造唾液和乳酸暴露的牙本质保护潜力。方法：本研究使用放射化学分析与偏振显微镜相结合。比较了在人类磨牙中MOD制剂中的不同应用。未处理的空腔和覆盖有可流动树脂的空腔用作对照。将48个标本浸入人工唾液（pH 7.5）中，将48个浸入乳酸（pH 4）中。在第1天到第21天之间测量（32）P的活性，并估计矿化组织的损失。结果：与阴性对照相比，在乳酸中，单键，双键或键加腔体封闭剂密封的腔体可显着降低矿化组织的损失（p <0.05）。唾液和乳酸都暴露后，粘合加封闭剂的应用被证明是最具保护性的治疗方法（p <0.05）。结论：基于这些体外研究结果，建议使用填充型腔封闭剂或可流动的树脂来保护牙本质免于脱矿质。放射化学分析对于评估粘合剂对牙本质脱矿质的保护潜力似乎很有价值。

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