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首页> 外文期刊>Journal of dermatological science >Topical application of laminin-332 to diabetic mouse wounds.
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Topical application of laminin-332 to diabetic mouse wounds.

机译：层粘连蛋白332在糖尿病小鼠伤口上的局部应用。

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BACKGROUND: Keratinocyte migration is essential for wound healing and diabetic wound keratinocytes migrate poorly. Keratinocyte migration and anchorage appears to be mediated by laminin-332 (LM-332). Impaired diabetic wound healing may be due to defective LM-332 mediated keratinocyte migration. OBJECTIVE: To evaluate LM-332 expression in diabetic (db/db) and control (db/-) mice and to test LM-332 wound healing effects when applied to mouse wounds. METHODS: LM-332 expression in mouse wounds was evaluated using immunohistochemistry. LM-332 wound healing effects were evaluated by directly applying soluble LM-332, a LM-332 biomaterial, or a control to mouse wounds. Percent wound closure and histology score, based on healing extent, were measured. RESULTS: Precursor LM-332 expression was markedly reduced in db/db when compared to db/- mice. In vitro, soluble LM-332 and LM-332 biomaterial demonstrated significant keratinocyte adhesion. In vivo, soluble LM-332 treated wounds had the highest histology score, but significant differences were not found between wound treatments (p>0.05). No differences in percentage wound closure between treatment and control wounds were found (p>0.05). CONCLUSION: The db/db wounds express less precursor LM-332 when compared to db/-. However, LM-332 application did not improve db/db wound healing. LM-332 purified from keratinocytes was primarily physiologically cleaved LM-332 and may not regulate keratinocyte migration. Application of precursor LM-332 rather than cleaved LM-332 may be necessary to improve wound healing, but this isoform is not currently available in quantities sufficient for testing.
机译：背景：角质形成细胞迁移对于伤口愈合必不可少，而糖尿病伤口角质形成细胞迁移能力差。角质形成细胞的迁移和锚定似乎是由层粘连蛋白332（LM-332）介导的。糖尿病伤口愈合不良可能是由于LM-332介导的角质形成细胞迁移缺陷所致。目的：评估LM-332在糖尿病（db / db）和对照（db /-）小鼠中的表达，并测试应用于小鼠伤口的LM-332伤口的愈合效果。方法：采用免疫组织化学方法检测小鼠伤口中LM-332的表达。通过将可溶性LM-332，LM-332生物材料或对照直接应用于小鼠伤口来评估LM-332的伤口愈合效果。根据愈合程度测量伤口闭合百分比和组织学评分。结果：与db /-小鼠相比，前体LM-332在db / db中的表达明显降低。在体外，可溶性LM-332和LM-332生物材料表现出明显的角质形成细胞粘附。在体内，可溶性LM-332处理的伤口具有最高的组织学评分，但在伤口处理之间未发现显着差异（p> 0.05）。在治疗伤口和对照伤口之间没有发现伤口闭合百分比的差异（p> 0.05）。结论：与db /-相比，db / db伤口表达的前体LM-332更少。但是，LM-332的应用并未改善db / db伤口的愈合。从角质形成细胞纯化的LM-332主要是在生理学上切割的LM-332，可能不调节角质形成细胞的迁移。可能需要使用前体LM-332而不是裂解的LM-332来改善伤口的愈合，但是这种同工型目前尚不足以用于测试。

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