...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dermatological science >The effects of epigallocatechin-3-gallate on extracellular matrix metabolism.
【24h】

The effects of epigallocatechin-3-gallate on extracellular matrix metabolism.

机译：表没食子儿茶素-3-没食子酸酯对细胞外基质代谢的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Anti-oxidants have attracted a lot of interest on account of their function to protect the skin from oxidative stress by ultraviolet (UV) radiation. OBJECTIVE: This study examined the effects of epigallocatechin-3-gallate (EGCG), which is a green tea extract, on the extracellular matrix (ECM) changes induced by UV radiation and showed the comparative results with retinoic acid (RA). METHODS: The ECM metabolism is tightly controlled by the collagen degrading matrix metalloprotienases (MMPs) and their tissue inhibitors (TIMPs). Therefore, the expression of MMPs and TIMP-1 was investigated to evaluate the effects of EGCG and RA. Artificial skin was made using three-dimensionally cultured keratinocytes on a collagen matrix populated with fibroblasts. EGCG and RA were added into the medium of the fibroblasts and keratinocytes culture and also applied topically on artificial skins prior to UVA irradiation. The MMPs and TIMP-1 expression levels were measured using Western blot and a zymogram. RESULTS: EGCG, like RA, decreased the level of MMPs production and increased TIMP-1 expression level. However, EGCG suppressed the activities of the gelatinases and augmented the expressions of the TIMP-1 more than RA did. RA decreased the MMP-1 and MMP-3 expression levels to a greater extent than EGCG. ECM alterations as a result of UVA appeared to be prevented more effectively using the EGCG treatment. CONCLUSION: EGCG can reverse the ECM degradation induced by UV even with a topical application of a practical-use concentration. In particular, EGCG proved to be much more effective in ROS-related conditions, such as UVA exposure.
机译：背景技术：抗氧化剂由于其保护皮肤免受紫外线（UV）辐射所产生的氧化应激的作用，引起了人们的极大兴趣。目的：本研究检查了绿茶提取物表没食子儿茶素-3-没食子酸酯（EGCG）对紫外线辐射诱导的细胞外基质（ECM）变化的影响，并显示了与维甲酸（RA）的比较结果。方法：ECM代谢受胶原降解基质金属金属蛋白酶（MMPs）及其组织抑制剂（TIMPs）严格控制。因此，研究了MMP和TIMP-1的表达以评估EGCG和RA的作用。使用三维培养的角质形成细胞在成纤维细胞组成的胶原蛋白基质上制成人造皮肤。将EGCG和RA加入成纤维细胞和角质形成细胞培养基中，并在UVA照射前局部施用于人造皮肤上。使用蛋白质印迹和酶谱图测量MMP和TIMP-1表达水平。结果：EGCG像RA一样，降低了MMPs的产生，并增加了TIMP-1的表达。但是，EGCG比RA抑制明胶酶的活性并增加TIMP-1的表达。 RA比EGCG降低MMP-1和MMP-3的表达水平更大。使用EGCG治疗似乎可以更有效地预防由于UVA引起的ECM改变。结论EGCG可以逆转由紫外线引起的ECM降解，即使局部应用实际使用浓度也是如此。特别地，事实证明，EGCG在与ROS有关的条件下（如UVA暴露）更为有效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号