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首页> 外文期刊>Journal of dermatological science >Changes in epidermal hyaluronan metabolism following UVB irradiation.
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Changes in epidermal hyaluronan metabolism following UVB irradiation.

机译：UVB照射后表皮透明质酸代谢的变化。

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BACKGROUND: Hyaluronan (HA) plays a role in keratinocyte proliferation and differentiation, and have shown different biological activities depending on its molecular mass. While many studies have shown changes in the amount of HA after UVB irradiation, molecular mass change remains to be elucidated. OBJECTIVE: To investigate the change in the molecular mass of HA after UVB irradiation in mouse epidermis. METHODS: The mice were irradiated with a single dose of UVB (0.15J/cm(2)). The amount of HA was examined using HABP sandwich assay. The molecular mass distribution was estimated by Sephacryl S-1000 chromatography. Has and Hyal mRNA expressions were detected by real-time PCR. RESULTS: On day 2 after UVB irradiation, both the amount of HA and the up-regulation of Has3 mRNA expression reached their maximum. The average HA molecular mass was about 1000 kDa, a level similar to that of the non-irradiated epidermis. On day 3, the average HA molecular mass drastically decreased to 100 kDa, while Hyal1, Hyal2, and Hyal3 mRNA expressions slightly increased. The amount of HA, however, remained high. On days 4 and 5, the amount of HA gradually decreased, but the molecular mass of HA remained low. A drastic reduction of the HA molecular mass after UVB irradiation was confirmed. CONCLUSION: UVB irradiation elicits remarkable changes in the molecular mass of HA, as well as amount. These qualitative and quantitative changes of HA might play an important role in UVB-induced cell proliferation and differentiation. Further study will be required to resolve the mechanism of HA degradation in the epidermis.
机译：背景：透明质酸（HA）在角质形成细胞的增殖和分化中发挥作用，并根据其分子量表现出不同的生物学活性。尽管许多研究表明，UVB照射后HA的量会发生变化，但分子量变化仍有待阐明。目的：探讨UVB照射后小鼠表皮中HA的分子量变化。方法：用单剂量的UVB（0.15J / cm（2））照射小鼠。使用HABP夹心测定法检查HA的量。通过Sephacryl S-1000色谱法估计分子量分布。通过实时PCR检测Has和Hyal mRNA表达。结果：UVB照射后第2天，HA的量和Has3 mRNA表达的上调均达到最大。 HA的平均分子量约为1000 kDa，与未经辐照的表皮相似。在第3天，平均HA分子量急剧下降至100 kDa，而Hyal1，Hyal2和Hyal3 mRNA表达略有增加。但是，HA的数量仍然很高。在第4和5天，HA的量逐渐减少，但是HA的分子量仍然较低。证实了UVB照射后HA分子量的急剧降低。结论：UVB照射引起HA的分子质量和数量的显着变化。 HA的这些定性和定量变化可能在UVB诱导的细胞增殖和分化中起重要作用。需要进一步的研究来解决表皮中HA降解的机制。

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