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首页> 外文期刊>Journal of dermatological science >Expression of mineralized tissue associated proteins: dentin sialoprotein and phosphophoryn in rodent hair follicles.
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Expression of mineralized tissue associated proteins: dentin sialoprotein and phosphophoryn in rodent hair follicles.

机译：矿化的组织相关蛋白：啮齿动物毛囊中的牙本质唾液蛋白和磷蛋白的表达。

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BACKGROUND: Mammalian hair development and tooth development are controlled by a series of reciprocal epithelial-mesenchymal interactions. Similar growth factors and transcription factors, such as fibroblast growth factor (FGF), sonic hedgehog homolog (SHH), bone morphogenetic proteins (BMPs) and Wnt10a, were reported to be involved in both of these interactions. Dentin sialoprotein (DSP) and phosphophoryn (PP) are the two major non-collagenous proteins secreted by odontoblasts that participate in dentin mineralization during tooth development. Because of striking similarities between tooth development and hair follicle development, we investigated whether DSP and/or PP proteins may also play a role in hair follicle development. OBJECTIVE: In this study, we examined the presence and location of DSP/PP proteins during hair follicle development. METHODS: Rat PP proteins were detected using immunohistochemical/immunofluorescent staining. DSP-PP mRNAs were detected by in situ hybridization with riboprobes. LacZ expression was detected in mouse tissues using a DSP-PP promoter-driven LUC in transgenic mice. RESULTS: We found that PP proteins and DSP-PP mRNAs are present in rat hair follicles. We also demonstrate that an 8 kb DSP-PP promoter is able to drive lacZ expression in hair follicles. CONCLUSION: We have firmly established the presence of DSP/PP in mouse and rat hair follicles by immunohistochemical/immunofluorescent staining, in situ hybridization with riboprobes and transgenic mice studies. The expression of DSP/PP in hair follicles is the first demonstration that major mineralization proteins likely may also contribute to soft tissue development. This finding opens a new avenue for future investigations into the molecular-genetic management of soft tissue development.
机译：背景：哺乳动物的头发发育和牙齿发育受一系列相互的上皮-间质相互作用的控制。据报道，类似的生长因子和转录因子，例如成纤维细胞生长因子（FGF），声波刺猬同源物（SHH），骨形态发生蛋白（BMP）和Wnt10a，都参与了这两种相互作用。牙本质唾液蛋白（DSP）和磷光蛋白（PP）是成牙本质细胞分泌的两种主要的非胶原蛋白，它们参与牙齿发育过程中的牙本质矿化。由于牙齿发育和毛囊发育之间惊人的相似性，我们研究了DSP和/或PP蛋白是否也可能在毛囊发育中起作用。目的：在这项研究中，我们检查了毛囊发育过程中DSP / PP蛋白的存在和位置。方法：采用免疫组织化学/免疫荧光染色法检测大鼠PP蛋白。通过与核糖核酸原位杂交检测DSP-PP mRNA。在转基因小鼠中使用DSP-PP启动子驱动的LUC在小鼠组织中检测到LacZ表达。结果：我们发现PP蛋白和DSP-PP mRNA存在于大鼠毛囊中。我们还证明了8 kb DSP-PP启动子能够驱动毛囊中的lacZ表达。结论：我们通过免疫组织化学/免疫荧光染色，与核糖核酸的原位杂交和转基因小鼠研究，已经牢固地建立了DSP / PP在小鼠和大鼠毛囊中的存在。 DSP / PP在毛囊中的表达是主要矿物质蛋白也可能有助于软组织发育的第一个证明。这一发现为软组织发育的分子遗传学管理的未来研究开辟了一条新途径。

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