• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dermatological science >Visualizing radiofrequency-skin interaction using multiphoton microscopy in vivo
【24h】

Visualizing radiofrequency-skin interaction using multiphoton microscopy in vivo

机译：在体内使用多光子显微镜可视化射频-皮肤相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Redundant skin laxity is a major feature of aging. Recently, radiofrequency has been introduced for nonablative tissue tightening by volumetric heating of the deep dermis. Despite the wide range of application based on this therapy, the effect of this technique on tissue and the subsequent tissue remodeling have not been investigated in detail. Objective: Our objective is to evaluate the potential of non-linear optics, including multiphoton autofluorescence and second harmonic generation (SHG) microscopy, as a non-invasive imaging modality for the real-time study of radiofrequency-tissue interaction. Methods: Electro-optical synergy device (ELOS) was used as the radiofrequency source in this study. The back skin of nude mouse was irradiated with radiofrequency at different passes. We evaluated the effect on skin immediately and 1 month after treatment with multiphoton microscopy. Results: Corresponding histology was performed for comparison. We found that SHG is negatively correlated to radiofrequency passes, which means that collagen structural disruption happens immediately after thermal damage. After 1 month of collagen remodeling, SHG signals increased above baseline, indicating that collagen regeneration has occurred. Our findings may explain mechanism of nonablative skin tightening and were supported by histological examinations. Conclusions: Our work showed that monitoring the dermal heating status of RF and following up the detailed process of tissue reaction can be imaged and quantified with multiphoton microscopy non-invasively in vivo.
机译：背景：皮肤松弛现象是衰老的主要特征。近来，已经通过深部真皮的体积加热将射频引入用于非消融性组织紧缩。尽管基于该疗法的广泛应用，但尚未详细研究该技术对组织的影响以及随后的组织重塑。目的：我们的目的是评估非线性光学的潜力，包括多光子自发荧光和二次谐波生成（SHG）显微镜，作为一种实时研究射频组织相互作用的非侵入性成像方式。方法：本研究以电光协同装置（ELOS）为射频源。裸鼠的背部皮肤以不同的频率被射频照射。我们立即评价了多光子显微镜治疗后以及治疗后1个月对皮肤的影响。结果：进行了相应的组织学比较。我们发现SHG与射频传递呈负相关，这意味着胶原蛋白结构破坏在热损伤后立即发生。胶原蛋白重塑1个月后，SHG信号增加至基线以上，表明发生了胶原蛋白再生。我们的发现可以解释非消融性皮肤紧缩的机制，并得到组织学检查的支持。结论：我们的工作表明，利用多光子显微镜在体内无创地监测RF的皮肤加热状态并跟踪组织反应的详细过程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号