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首页> 外文期刊>Journal of dermatological science >c-JUN N-terminal kinase-1 (JNK1) but not JNK2 or JNK3 is involved in UV signal transduction in human epidermis.
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c-JUN N-terminal kinase-1 (JNK1) but not JNK2 or JNK3 is involved in UV signal transduction in human epidermis.

机译：c-JUN N末端激酶1（JNK1）但不参与人表皮的UV信号转导JNK2或JNK3。

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摘要

BACKGROUND: c-Jun N-terminal kinase (JNK) plays a critical role in UV-induced apoptotic cell death. Although three isoforms are known in mammals, physiological roles of each isoform are still obscure. Furthermore, our recent findings show that serpin squamous cell carcinoma antigen (SCCA1) binds to JNK. OBJECTIVE: To determine which isoform is responsible for the UV signal transduction in human epidermis and whether SCCA1 is capable to regulate kinase activity of a specific isoform. METHODS: Immunohistochemical localization of each JNK isoform was investigated after UV irradiation in vivo and in vitro. Effect of recombinant SCCA1 on JNK kinase activity was also analyzed. RESULTS: Immunostaining for JNK1, 2 and 3 demonstrated marked elevation of JNK1 in spinous to granular cells of UV-irradiated skin, whereas they were expressed weakly in upper epidermis of the sun-protected, buttock skin. In cultured keratinocytes, only JNK1 is translocated into nucleus after UV irradiation. JNK2, which localized in the cytoplasm, or JNK3, which was confined in nucleus, remained in the same compartment after UV irradiation. We confirmed that only JNK1 mRNA was up-regulated after UV irradiation in cultured keratinocytes. In addition, recombinant SCCA1 suppressed kinase activity of JNK1 but did not affect JNK2 or JNK3 kinase activity. CONCLUSION: JNK1 is associated with UV signal transduction in human epidermis and SCCA1 is a suppressor of this process.
机译：背景：c-Jun N末端激酶（JNK）在紫外线诱导的凋亡细胞死亡中起关键作用。尽管在哺乳动物中已知三种同工型，但是每种同工型的生理作用仍然不清楚。此外，我们最近的发现表明，丝氨酸蛋白酶抑制鳞状上皮细胞癌抗原（SCCA1）与JNK结合。目的：确定哪种同工型负责人表皮中的紫外线信号转导，以及SCCA1是否能够调节特定同工型的激酶活性。方法：在体内和体外进行紫外线照射后，研究了每种JNK同工型的免疫组织化学定位。还分析了重组SCCA1对JNK激酶活性的影响。结果：JNK1、2和3的免疫染色显示，在受紫外线照射的皮肤的棘突到颗粒细胞中，JNK1明显升高，而在受防晒保护的臀部皮肤的上表皮中则弱表达。在培养的角质形成细胞中，紫外线照射后只有JNK1易位到核中。紫外线照射后，位于细胞质中的JNK2或局限在细胞核中的JNK3仍留在同一隔室中。我们证实，在培养的角质形成细胞中，紫外线照射后仅JNK1 mRNA上调。此外，重组SCCA1抑制JNK1的激酶活性，但不影响JNK2或JNK3激酶的活性。结论：JNK1与人表皮中的紫外线信号转导有关，而SCCA1是该过程的抑制剂。

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