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首页> 外文期刊>Journal of Electroanalytical Chemistry: An International Journal Devoted to All Aspects of Electrode Kinetics, Interfacial Structure, Properties of Electrolytes, Colloid and Biological Electrochemistry >Magnetic glass carbon electrode, modified with magnetic ferriferrous oxide nanoparticles coated with molecularly imprinted polymer films for electrochemical determination of bovine hemoglobin
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Magnetic glass carbon electrode, modified with magnetic ferriferrous oxide nanoparticles coated with molecularly imprinted polymer films for electrochemical determination of bovine hemoglobin

机译：磁性玻璃碳电极，用磁性三氧化二铁纳米粒子修饰并涂有分子印迹聚合物薄膜，用于电化学测定牛血红蛋白

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摘要

The modified electrode based on magnetic ferriferrous oxide (Fe_3O_4) nanoparticles covered with molecularly imprinted polymer was fabricated and used for electrochemical recognition of bovine hemoglobin(BHb). First, the magnetic Fe_3O_4 nanoparticles were covered with silicon dioxide. Then dopamine (DA) was self-polymerized on the surface of magnetic nanoparticles in the presence of bovine hemoglobin as the template molecular. The molecularly imprinted polymers were characterized by transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FT-IR) and electrochemical impedance spectroscopy (EIS). Potassium hexacyanoferrate (K_3[Fe(CN)_6]) was used as an electroactive probe for measurement signal. Under optimized conditions (70 min for adsorption, pH = 6.0), the DPV peak current regressed linearly with the BHb concentration increase in the range of 5.0×10~(-7) to 1.0×10~(-5)gmL~(-1)(r = 0.9939) with a detection limit of 1.184×10~(-8) gmL~(-1) (S/N = 3). Several proteins were tested to confirm the imprinting effect of the modified electrode. The results showed the good selectivity of the resulting biosensors. The applicability of the method for complex matrix analysis was also evaluated and the modified electrode showed good stability and reproducibility.
机译：制备了基于分子印迹聚合物覆盖的磁性三氧化二铁（Fe_3O_4）纳米粒子的修饰电极，并将其用于电化学识别牛血红蛋白（BHb）。首先，磁性Fe_3O_4纳米颗粒被二氧化硅覆盖。然后在牛血红蛋白作为模板分子的存在下，多巴胺（DA）在磁性纳米颗粒的表面上自聚合。分子印迹聚合物通过透射电子显微镜（TEM），傅立叶变换红外光谱（FT-IR）和电化学阻抗谱（EIS）进行表征。六氰合铁酸钾（K_3 [Fe（CN）_6]）用作测量信号的电活性探针。在最佳条件下（吸附70分钟，pH = 6.0），DPV峰值电流随BHb浓度在5.0×10〜（-7）至1.0×10〜（-5）gmL〜（-）范围内线性回归。 1）（r = 0.9939），检出限为1.184×10〜（-8）gmL〜（-1）（S / N = 3）。测试了几种蛋白质以确认修饰电极的印迹效果。结果表明所得生物传感器具有良好的选择性。还评估了该方法在复杂基质分析中的适用性，修饰电极显示出良好的稳定性和可重复性。

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